EXCITATION-CONTRACTION IN ISOLATED CARDIAC CELLS

我们分离了成年大鼠和兔的心肌细胞。 这些制剂正被用来研究收缩， 电生理和生化特性的各种 不同的条件。特别是，我们已经能够在这些 孤立细胞：一种自发产生的纵向传播波 在静息膜电位正常的细胞中 是电刺激的。这些“浪潮”很可能代表着 肌浆自发性钙释放现象 网状结构(SR)是导致散射光强度的原因 我们实验室过去研究过的波动(SLIF) 多细胞制剂。通过我们的工作，我们初步验证了 以单细胞为模型研究血管紧张素转化机制 兴奋-收缩耦合，特别是自发性肌浆网钙 放手。我们最近扩展了我们的工作以研究相互作用 在自发的收缩波和刺激的抽搐之间。

We have dissociated myocardial muscle cells from adult rats and rabbits. These preparations are being used to study the contractile, electrophysiological, and biochemical characteristics in a variety of different conditions. In particular we have been able to identify in these isolated cells a longitudinally propagating wave which occurs spontaneously in cells with a normal resting membrane potential which are not electrically stimulated. These "waves" are likely to represent the phenomenon of spontaneous release of calcium from the sarcoplasmic reticulum (SR) and are the cause of the scattered light intensity fluctuations (SLIF) which our laboratory has studied in the past in multicellular preparations. With our work we have initially validated single cells as a model for the study of mechanisms of excitation-contraction coupling and in particular of spontaneous SR calcium release. We have recently expanded our work to study the interaction between spontaneous contractile waves and stimulated twitches.