EXCITATION-CONTRACTION IN ISOLATED CARDIAC CELLS

我们分离了成年大鼠和兔的心肌细胞。 这些制剂正被用来研究收缩， 电生理和生化特性的各种 不同的条件。特别是，我们已经能够在这些 孤立细胞：一种自发产生的纵向传播波 当细胞没有受到电刺激并且被认为处于 静息，并具有正常的静息膜电位。这些“浪潮”很可能是 代表钙诱导的钙释放(CICR)现象和 是散射光强度起伏(SLIF)的原因 我们的实验室过去曾研究过多细胞制剂。使用 我们最初的工作是尝试将单个细胞作为模型进行验证 用于研究兴奋-收缩耦合的机制和In 特别是CICR。随后我们测量了(1)的效果。 电刺激；(2)对自发波的影响。

We have dissociated myocardial muscle cells from adult rats and rabbits. These preparations are being used to study the contractile, electrophysiological, and biochemical characteristics in a variety of different conditions. In particular we have been able to identify in these isolated cells a longitudinally propagating wave which occurs spontaneously when the cell is not being electrically stimulated and is considered at rest and has a normal resting membrane potential. These "waves" are likely to represent the phenomenon of calcium induced calcium release (CICR) and are the cause of the scattered light intensity fluctuations (SLIF) which our laboratory has studied in the past in multicellular preparations. With our work we have initially attempted to validate single cells as a model for the study of mechanisms of excitation-contraction coupling and in particular of CICR. Subsequently we have measured the effect of (1) electrical stimulation and (2) on the spontaneous waves.