BIOCHEMICAL BASIS FOR DEFECTIVE DIFFERENTIATION IN GRANULOCYTIC LEUKEMIA

粒细胞白血病分化缺陷的生化基础

• 批准号: 3817352
• 负责人: W H EVANS
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3817352
• 关键词: blood; bone marrow; cell components; cell differentiation; cellular oncology; diagnosis design /evaluation; early diagnosis; granulocyte; host neoplasm interaction; human subject; humoral immunity; myelogenous leukemia; neoplasm /cancer genetics; neoplasm /cancer immunodiagnosis; tissue /cell culture

The main thrust of this work is to develop biochemical methods for the early diagnosis of granulocytic leukemia and methods for inducing leukemic cells to develop some or all of their functional properties as a means of partially or completely restoring host defense mechanisms in leukemia patients. Work is first aimed at establishing which of the many biochemical steps involved in normal granulocyte differentiation are controlled by humoral regulators. The results will be compared with those obtained from similar studies on leukemic cells at corresponding stages of maturity in order to determine, the nature and potential reversibility of the arrested differentiation steps. Biochemical analyses are carried out on mature and immature granulocytes isolated from blood and bone marrow and the effects of cell regulators on granulocyte differentiation, as measured by changes in the synthesis of specific cellular components, are studied in a defined culture system previously developed in this laboratory. Possible relationships between transforming genes in leukemic myeloblasts and factors involved in the regulation of normal granulocyte differentiation are under investigation.

这项工作的主旨是发展生物化学方法， 粒细胞白血病早期诊断及方法 诱导白血病细胞发展出它们的部分或全部功能 属性作为部分或完全恢复主机的方法 白血病患者的防御机制 工作首先针对 确定在正常情况下， 粒细胞分化受体液调节剂控制。 结果将与类似的实验结果进行比较。 在相应成熟阶段的白血病细胞的研究， 为了确定，性质和潜在的可逆性， 阻止分化步骤。 进行生化分析， 从血液中分离出成熟和未成熟的粒细胞， 骨髓及细胞调节剂对粒细胞的影响 分化，如通过合成的变化来测量， 特定的细胞成分，在确定的培养物中进行研究 这个实验室以前开发的系统。 可能 白血病成髓细胞转化基因的关系 以及参与正常粒细胞调节的因子 差异正在调查中。