THE BIOCHEMICAL BASIS FOR DEFECTIVE DIFFERENTIATION IN GRANULOCYTIC LEUKEMIA

粒细胞白血病分化缺陷的生化基础

• 批准号: 3962889
• 负责人: W H EVANS
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3962889
• 关键词: blood; bone marrow; cell components; cell differentiation; cellular oncology; density gradient ultracentrifugation; early diagnosis; electron microscopy; gas chromatography; gel electrophoresis; granulocyte; host neoplasm interaction; human tissue; humoral immunity; immunochemistry; immunofluorescence technique; immunologic techniques; leukopoiesis; mass spectrometry; myelogenous leukemia; myeloid stem cell; neoplasm /cancer immunodiagnosis; neoplasm /cancer immunology; neoplasm /cancer transplantation; neoplastic cell; oncogenes; phagocytosis; radioimmunoassay; radiotracer; scintillation counter; thin layer chromatography; tissue /cell culture

The main thrust of this work is to develop biochemical methods for the early diagnosis of granulocytic leukemia and methods for inducing leukemic cells to develop some or all of their functional properties as a means of partially or completely restoring host defense mechanisms in leukemia patients. Work is first aimed at establishing which of the many biochemical steps involved in normal granulocyte differentiation are controlled by humoral regulators. The results will be compared with those obtained from similar studies on leukemic cells at corresponding stages of maturity in order to determine the nature and potential reversibility of the arrested differentiation steps. Biochemical analyses are carried out on mature and immature granulocytes isolated from blood and bone marrow and the effects of external cell regulators on granulocyte differentiation, as measured by changes in the synthesis of specific cellular components, are studied in a defined culture system previously developed in this laboratory. Possible relationships between transforming genes in leukemic myeloblasts and factors involved in the regulation of normal granulocyte differentiation are under investigation.

这项工作的主要目的是开发用于 粒细胞白血病的早期诊断和诱发白血病的方法 细胞发展其部分或全部功能特性作为一种手段 部分或完全恢复白血病宿主防御机制 患者。 工作首先旨在确定众多因素中的哪一个 正常粒细胞分化涉及的生化步骤是 由体液调节剂控制。 结果将与那些进行比较 从相应阶段的白血病细胞的类似研究中获得 成熟度以确定其性质和潜在可逆性 被阻止的分化步骤。 进行生化分析 对从血液和骨髓中分离出的成熟和未成熟粒细胞的影响 外部细胞调节剂对粒细胞分化的影响，如 通过特定细胞成分合成的变化来测量， 在先前开发的特定培养系统中进行研究 实验室。 白血病转化基因之间可能存在的关系 成髓细胞和参与正常粒细胞调节的因子 差异化正在研究中。