BIOCHEMICAL BASIS FOR DEFECTIVE DIFFERENTIATION IN GRANULOCYTIC LEUKEMIA

粒细胞白血病分化缺陷的生化基础

• 批准号: 3939187
• 负责人: W H EVANS
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3939187
• 关键词: blood; bone marrow; cell components; cell differentiation; cellular oncology; early diagnosis; granulocyte; host neoplasm interaction; human tissue; humoral immunity; myelogenous leukemia; myeloid stem cell; neoplasm /cancer immunodiagnosis; neoplasm /cancer immunology; phagocytosis; tissue /cell culture

The main thrust of this work is to develop biochemical methods for the early diagnosis of granulocytic leukemia and methods for inducing leukemic cells to develop some or all of their functional properties as a means of partially or completely restoring host defense mechanism in leukemia patients. Work is first aimed at establishing which of the many biochemical steps involved in normal granulocyte differentiation are controlled by humoral regulators. The results will be compared with those obtained from similar studies on leukemic cells at corresponding stages of maturity in order to determine the nature and potential reversibility of the arrested differentiation steps. Biochemical analyses are carried out on mature and immature granulocyte isolated from blood and bone marrow and the effects of external cell regulators on granulocyte differentiation, as measured by changes in the synthesis of specific cellular components, are studied in a defined cultured system previously developed in this laboratory. Possible relationships between transforming genes in leukemic myeloblasts and factors involved in the regulation of normal granulocyte differentiation are under investigation.

这项工作的主旨是发展生物化学方法 用于粒细胞白血病的早期诊断以及用于 诱导白血病细胞发展其部分或全部功能 属性作为部分或完全恢复主机的方法 白血病患者的防御机制 工作首先针对 确定参与的许多生化步骤中 正常的粒细胞分化受体液调节， 监管部门 结果将与获得的结果进行比较 从对相应阶段的白血病细胞的类似研究中， 成熟度，以确定性质和潜力 阻止分化步骤的可逆性。 生化 对成熟和未成熟的粒细胞进行分析， 从血液和骨髓中分离出来以及外部的影响 细胞调节剂对粒细胞分化的影响，如通过 特定细胞成分合成的变化， 在以前开发的一个确定的培养系统中进行了研究， 实验室 转化基因之间的可能关系 白血病成髓细胞和参与调节的因子 正常粒细胞分化正在研究中。