THE BIOCHEMICAL BASIS FOR DEFECTIVE DIFFERENTIATION IN GRANULOCYTIC LEUKEMIA

粒细胞白血病分化缺陷的生化基础

• 批准号: 4691689
• 负责人: W H EVANS
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4691689
• 关键词: blood; bone marrow; cell components; cell differentiation; cellular oncology; density gradient ultracentrifugation; early diagnosis; electron microscopy; gas chromatography; gel electrophoresis; granulocyte; host neoplasm interaction; human tissue; humoral immunity; immunochemistry; immunofluorescence technique; immunologic techniques; leukopoiesis; mass spectrometry; myelogenous leukemia; myeloid stem cell; neoplasm /cancer immunodiagnosis; neoplasm /cancer immunology; neoplasm /cancer transplantation; neoplastic cell; oncogenes; phagocytosis; radioimmunoassay; radiotracer; scintillation counter; thin layer chromatography; tissue /cell culture

The main thrust of this work is to develop biochemical methods for the early diagnosis of granulocytic leukemia and methods for inducing leukemic cells to develop some or all of their functional properties as a means of partially or completely restoring host defense mechanisms in leukemia patients. Work is first aimed at establishing which of the many biochemical steps involved in normal granulocyte differentiation are controlled by humoral regulators. The results will be compared with those obtained from similar studies on leukemic cells at corresponding stages of maturity in order to determine the nature and potential reversibility of the arrested differentiation steps. Biochemical analyses are carried out on mature and immature granulocytes isolated from blood and bone marrow and the effects of external cell regulators on granuloctye differentiation, as measured by changes in the synthesis of specific cellular components, are studied in defined culture sytems previously developed in this laboratory. Possible relationships between transforming genes in leukemic myeloblasts and factors involved in the regulation of normal granulocyte differentiation are under investigation.

这项工作的主旨是开发用于生物化学的方法 粒细胞白血病的早期诊断及诱发方法 细胞发展它们的部分或全部功能特性作为一种手段 部分或完全恢复白血病的宿主防御机制 病人。首先，工作的目的是确定众多 参与正常粒细胞分化的生化步骤有 由体液调节器控制。结果将与那些 从对相应阶段白血病细胞的类似研究中获得 成熟度以确定其性质和潜在的可逆性 受阻的分化步骤。进行生化分析 从血液和骨髓中分离的成熟和未成熟的粒细胞和 外源细胞调节剂对粒细胞分化的影响 通过特定细胞成分合成的变化来衡量，是 在本实验室先前开发的已定义的培养系统中进行研究。 白血病成髓细胞转化基因间的可能关系 以及与正常粒细胞调节有关的因素 分化正在调查中。