THE BIOCHEMICAL BASIS FOR DEFECTIVE DIFFERENTIATION IN GRANULOCYTIC LEUKEMIA

这项工作的主旨是发展生物化学方法， 粒细胞白血病的早期诊断和诱导白血病的方法 细胞发展其部分或全部功能特性作为一种手段， 部分或完全恢复白血病中的宿主防御机制 患者 工作的目的首先是确定哪些许多 涉及正常粒细胞分化的生化步骤是 由体液调节剂控制。 结果将与那些 从对相应阶段的白血病细胞的类似研究中获得， 到期日，以确定的性质和潜在的可逆性 停止分化的步骤。 进行生化分析 从血液和骨髓中分离的成熟和未成熟粒细胞， 外部细胞调节剂对粒细胞分化的影响，如 通过特定细胞成分合成的变化来衡量， 在本实验室先前开发的特定培养系统中进行研究。 白血病成髓细胞转化基因的可能关系 以及参与正常粒细胞调节的因子 差异正在调查中。

The main thrust of this work is to develop biochemical methods for the early diagnosis of granulocytic leukemia and methods for inducing leukemic cells to develop some or all of their functional properties as a means of partially or completely restoring host defense mechanisms in leukemia patients. Work is first aimed at establishing which of the many biochemical steps involved in normal granulocyte differentiation are controlled by humoral regulators. The results will be compared with those obtained from similar studies on leukemic cells at corresponding stages of maturity in order to determine the nature and potential reversibility of the arrested differentiation steps. Biochemical analyses are carried out on mature and immature granulocytes isolated from blood and bone marrow and the effects of external cell regulators on granuloctye differentiation, as measured by changes in the synthesis of specific cellular components, are studied in defined culture sytems previously developed in this laboratory. Possible relationships between transforming genes in leukemic myeloblasts and factors involved in the regulation of normal granulocyte differentiation are under investigation.