BIOCHEMICAL BASIS FOR DEFECTIVE DIFFERENTIATION IN GRANULOCYTIC LEUKEMIA

这项工作的主旨是开发生物化学方法来 粒细胞白血病的早期诊断及治疗方法 诱导白血病细胞发展其部分或全部功能 属性作为部分或完全恢复主机的手段 白血病患者的防御机制。工作的第一个目标是 确定许多生化步骤中的哪些涉及正常 粒细胞分化是由体液调节因子控制的。 结果将与类似的结果进行比较。 中国人相应成熟阶段白血病细胞的研究 的性质和潜在的可逆性 分化步骤受阻。进行生化分析 从血液中分离出成熟和未成熟的粒细胞 骨髓及细胞调节剂对粒细胞的影响 分化，由合成过程中的变化来衡量 特定的细胞成分，在特定的培养条件下进行研究 该系统以前是在这个实验室开发的。可能的 白血病成髓细胞转化基因之间的关系 以及与正常粒细胞调节有关的因素 分化正在调查中。

The main thrust of this work is to develop biochemical methods for the early diagnosis of granulocytic leukemia and methods for inducing leukemic cells to develop some or all of their functional properties as a means of partially or completely restoring host defense mechanisms in leukemia patients. Work is first aimed at establishing which of the many biochemical steps involved in normal granulocyte differentiation are controlled by humoral regulators. The results will be compared with those obtained from similar studies on leukemic cells at corresponding stages of maturity in order to determine, the nature and potential reversibility of the arrested differentiation steps. Biochemical analyses are carried out on mature and immature granulocytes isolated from blood and bone marrow and the effects of cell regulators on granulocyte differentiation, as measured by changes in the synthesis of specific cellular components, are studied in a defined culture system previously developed in this laboratory. Possible relationships between transforming genes in leukemic myeloblasts and factors involved in the regulation of normal granulocyte differentiation are under investigation.