How simple plastic surfaces can be recruited to the fight against contact transmission of SARS-CoV-2

到目前为止，严重急性呼吸系统综合征冠状病毒2（SARS-CoV-2）似乎很容易通过空气中的飞沫和表面传播。早期的研究表明，与其他表面相比，病毒在某些表面上的传染性似乎更长。然而，目前尚不清楚表面化学在病毒在表面上的宿主外的存活、感染性和变性过程中起什么作用。简单的聚合物，其表面化学减少了周围环境中感染性病毒的转移，将具有优于目前可用的活性负载和蛋白质包被的产品的优势，这些产品随着时间的推移而耗尽和降解。本项目的目的是确定对病毒吸附、感染性和变性行为的影响; 1）一系列市售手套和其他PPE表面，例如护目镜，以能够推荐哪些现有聚合物应用于最佳PPE，和2）使用诺丁汉大学最近开发的用于选择杀病毒性能的高通量方法筛选新型聚合物表面，开发用于PPE和接触传播控制的新聚合物。目标：1。在环境条件下对现有PPE塑料的病毒结合和灭活进行定量。使用微阵列上的文库定量SARS-CoV-2病毒样颗粒（VLP）或含SARS 2刺突的假病毒（PV）与聚合物结合的程度和特异性。定量SARS-CoV-2病毒与载玻片或多孔板和其他PPE材料上按比例放大的“前10名”感兴趣聚合物结合的吸附、感染性和变性（使用诺丁汉的新设施）。开发用于PPE的抗SARS-CoV-2聚合物，并更广泛地用于感染防护控制表面。

To date, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) appears to spread easily in droplets in the air and via surfaces. Early work indicates that viruses appear to remain infectious longer on some surfaces compared to others. However, it is currently unclear what role the surface chemistry plays in the survival, infectivity and denaturation process of the virus outside a host on surfaces.Simple polymers, on which the surface chemistry reduces the transfer of infectious viruses in the ambient environment, would have the advantage over the currently available active-loaded and protein-coated products which deplete and degrade over time. The aims of this project are to identify the effect on the virus adsorption, infectivity and denaturation behaviours of; 1) a range of commercially available gloves and other PPE surfaces, e.g. visors, to enable a recommendation of which existing polymers should be employed for optimal PPE, and 2) screen novel polymer surfaces using high throughput methodologies for selection of virucidal performance recently developed at the University of Nottingham, to develop new polymers for PPE and contact transmission control.Objectives:1. Quantify viral binding and inactivation under ambient conditions to existing PPE plastics.2. Quantify the magnitude and specificity of SARS-CoV-2 virus-like particle (VLP) or SARS2 spike-containing pseudovirus (PV) binding to polymers using libraries presented on micro arrays.3. Quantify the adsorption, infectivity and denaturation of SARS-CoV-2 viruses bound to the scaled up 'top 10' polymers of interest on slide or multiwell-plate and other PPE materials (using new facility in Nottingham).4. Develop anti-SARS-CoV-2 polymers for PPE and more widely for infection protection control surfaces.