MUBANY - Mechanistically Understand the Bactericidal Action of Nanopillar Topography

受大自然启发的纳米柱表面展示了一种控制细菌在表面上定植的方法，而这正是这项研究的目的所在。这是尚未完全解决的基础机制，阻碍了新型抗菌表面的发展及其在实际应用中的实施。mubani旨在研究纳米柱表面与细菌细胞壁之间导致细胞死亡的相互作用动力学，重点是在纳米尺度上破译时间分辨的相互作用。首次利用拉曼光谱结合稳定同位素探测技术对表面附着细菌的代谢活性进行评估，并对细菌进行活/死筛选。该项目将在相关工作流程中结合互补的2D/3D成像模式套件和低温样品制备方法，推进当前最先进的实践。该方法将为阐明纳米柱形貌的杀菌机理提供基础数据。最有效的表面参数，以达到最佳的杀菌活性将确定使用制备纳米柱聚合物表面作为测试底物。该奖学金将使申请人能够进行原创和强大的研究计划，使他处于新兴生物纳米材料领域的前沿。广泛的培训计划将支持申请人获得先进纳米制造，相关显微镜，定量数据分析的研究技能，并提高他在沟通，拨款写作和项目管理方面的可转移技能。申请人将把他的专业网络扩展到英国和欧盟。这项研究的社会经济效益超出了明显的科学和健康成果，计划的公众参与活动提高了公民对多学科研究方法的认识，以应对抗菌素耐药性等重大全球挑战。

Nature-inspired nanopillared surfaces demonstrate a means by which bacterial colonisation on surfaces can be controlled, and to do so is what this research aims to discover. It is the underpinning mechanism that is not yet fully resolved, impeding the development of novel antimicrobial surfaces and their implementation in real-world applications. MUBANY aims to investigate the kinetics of interactions between nanopillar surfaces and bacterial cell wall leading to cell death, focusing on deciphering time-resolved interactions at nanometer scales. For the first time, the metabolic activity of the surface attached bacteria will be assessed by Raman spectroscopy coupled with stable isotope probing in correlation with live/dead screening of bacteria. The project will combine a complementary suite of 2D/3D imaging modalities in a correlative workflow with a cryogenic sample preparation method, advancing the current state-of-the-art practices. This approach will establish ground truth data to elucidate the bactericidal mechanism of nanopillar topography. The most effective surface parameters to achieve optimal bactericidal activity will be determined using fabricated nanopillar polymer surfaces as test substrates. This fellowship will enable the applicant to conduct an original and robust research program, placing him at the forefront of an emerging field of bio-nanomaterials. An extensive training program will support the applicant to gain research skills in advanced nanofabrication, correlative microscopy, quantitative data analysis and improve his transferrable skills in communication, grant writing, and project management. The applicant will extend his professional network to the UK and EU. The socio-economic benefits of this research extend beyond the obvious scientific and health outcomes with planned public engagement activities raising citizen awareness of multidisciplinary research approaches to combat critical global challenges like antimicrobial resistance.