In vitro and in vivo studies of 3D orthopaedic implants with cell-instructive nanotopographies

具有细胞指导性纳米拓扑结构的 3D 骨科植入物的体外和体内研究

• 批准号: MR/S010343/1
• 负责人: Bo Su
• 金额: $ 90.85万
• 依托单位: University of Bristol
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FS010343%2F1
• 关键词: vitro; vivo; studies; 3D; orthopaedic

The demand for total hip and knee replacements (THR & TKR) has been steadily increasing worldwide. This is due to an ageing population, the rise in obesity and a change to more active lifestyles; together, these factors are leading to more osteoarthritis and degeneration of cartilage and subchondral bone in joints. The two leading causes of this type of orthopaedic implant failure are loosening (due to poor bone contact with the implant) and infection (due to bacterial infiltration and biofilm formation). In the case of THR/TKR, the incidence rate of joint infections is reported to be 1%- 4% in primary surgery and up to 30% in revision surgery. Our previous work showed that certain nanostructured surfaces on titanium (many orthopaedic implants are made from titanium) surfaces could be both bactericidal and bioactive (bone forming). However, most of the work so far was carried out on nanostructured 2D surfaces rather than real implant surfaces.This project is aimed at conducting in vitro and pre-clinical studies based on 3D porous Ti implants with nanostructured surfaces, hence translating our current understanding in 2D into real 3D implants. We have assembled a multidisciplinary team from universities of Bristol, Glasgow and Cambridge, in partnership with clinical and industrial partners, to progress our research findings from previous EPSRC projects focusing on the basic science of bactericidal topographies on 2D surfaces towards the real world applications. We will bring together expertise in biomaterials, nanotechnology, stem cell biology, microbiology and pre-clinical studies, with a clear vision to apply new smart cell-instructive surfaces to orthopaedic implants. We hope to improve the quality of life of patients who require implants and save the costs of NHS by avoiding the need for revision due to implant loosening and infection.

全球范围内对全髋关节和全膝关节置换术（THR和TKR）的需求一直在稳步增长。这是由于人口老龄化，肥胖症的增加和更积极的生活方式的改变;这些因素共同导致更多的骨关节炎和关节软骨和软骨下骨的退化。这类骨科植入物失效的两个主要原因是松动（由于骨与植入物接触不良）和感染（由于细菌浸润和生物膜形成）。在THR/TKR的情况下，据报告初次手术中关节感染的发生率为1%- 4%，翻修手术中高达30%。我们以前的工作表明，钛（许多骨科植入物由钛制成）表面上的某些纳米结构表面可能具有杀菌和生物活性（骨形成）。然而，迄今为止，大多数工作都是在纳米结构的2D表面上进行的，而不是真实的植入物表面。本项目旨在基于具有纳米结构表面的3D多孔钛植入物进行体外和临床前研究，从而将我们目前对2D的理解转化为真实的3D植入物。我们已经组建了一个来自布里斯托，格拉斯哥和剑桥大学的多学科团队，与临床和工业合作伙伴合作，将我们以前的EPSRC项目的研究成果集中在二维表面上的杀菌拓扑结构的基础科学上，并将其应用于真实的世界。我们将汇集生物材料、纳米技术、干细胞生物学、微生物学和临床前研究方面的专业知识，以明确的愿景将新的智能细胞指导表面应用于骨科植入物。我们希望通过避免因植入物松动和感染而需要翻修来提高需要植入物的患者的生活质量并节省NHS的成本。

项目成果

Resolving physical interactions between bacteria and nanotopographies with focused ion beam scanning electron microscopy.

使用聚焦离子束扫描电子显微镜解决细菌和纳米拓扑结构之间的物理相互作用。

• DOI: 10.1016/j.isci.2021.102818
• 发表时间: 2021-07-23
• 期刊: iScience
• 影响因子: 5.8
• 作者: Jenkins J;Ishak MI;Eales M;Gholinia A;Kulkarni S;Keller TF;May PW;Nobbs AH;Su B
• 通讯作者: Su B

Enhanced and Stem-Cell-Compatible Effects of Nature-Inspired Antimicrobial Nanotopography and Antimicrobial Peptides to Combat Implant-Associated Infection.

• DOI: 10.1021/acsanm.2c04913
• 发表时间: 2023-02-24
• 期刊: ACS APPLIED NANO MATERIALS
• 影响因子: 5.9
• 作者: Ishak, Mohd Irill;Eales, Marcus;Damiati, Laila;Liu, Xiayi;Jenkins, Joshua;Dalby, Matthew J.;Nobbs, Angela H.;Ryadnov, Maxim G.;Su, Bo
• 通讯作者: Su, Bo