IDENTIFICATION AND CHARACTERIZATION OF A NOVAL PEPTIDE OSTEOCLASTPOIETIC FACTOR

新型肽破骨细胞生成因子的鉴定和表征

• 批准号: 3747914
• 负责人: TOSHIYUKI YONEDA
• 金额: --
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3747914
• 关键词: animal tissue; autoradiography; bioassay; bone metabolism disorder; chickens; cytokine; gene expression; genetic library; human subject; immunoaffinity chromatography; laboratory mouse; laboratory rat; molecular cloning; organ culture; osteoclast activating factor; osteoclasts; osteogenesis; pathologic bone resorption; physiologic bone resorption; polymerase chain reaction; protein purification; protein sequence

We have identified a novel factor which stimulates the formation of osteoclasts and which is produced by a human tumor as well as by leukocytes which have been stimulated with concanavalin A. We have called this peptide osteoclastpoietic factor (OPF). We have obtained amino acid sequence on this peptide and found that it is novel and distinct from other known growth regulatory factors, interleukins, and colony stimulating factors. We have found that a synthetic peptide which corresponds to this amino acid sequence mimics the biological effects, and we have produced both monoclonal and polyclonal antibodies which neutralize the original biological activity. In this project, we plan to: 1. Clone the gene and express the recombinant molecule. 2. Determine the role of OPF in normal bone remodeling and in disease states associated with increased bone resorption. Characterization of novel cytokines which are powerful stimulators of osteoclastic bone resorption is likely to lead to new insights in the normal control of osteoclast function and in the pathophysiology of disease states associated with bone destruction.

我们已经发现了一种新的因子，它刺激了 破骨细胞，其由人肿瘤以及由 用伴刀豆球蛋白A刺激的白细胞。我们称 破骨细胞生成因子（OPF）。 我们已经获得了氨基酸 序列，并发现它是新的和不同的 其它已知的生长调节因子、白细胞介素和集落 刺激因素。 我们已经发现， 对应于该氨基酸序列模拟生物学效应， 我们已经生产了单克隆和多克隆抗体， 中和了原有的生物活性 在这个项目中，我们计划 收件人： 1.克隆该基因并表达重组分子。 2.确定OPF在正常骨重建和疾病中的作用 与骨吸收增加相关的状态。 表征作为免疫调节剂的强刺激剂的新型细胞因子， 骨吸收 可能会带来新的见解， 破骨细胞功能的正常控制和 与骨质破坏相关的疾病状态。