EVALUATION OF FREE THYROXIN TECHNIQUES

最近，用于测量的非同位素(通常是自动化的)免疫分析 血清游离甲状腺素(FT4)已经可用。虽然成本更高 与放射免疫分析相比，它们要方便得多。我们 研究内源性白蛋白对甲状腺激素结合的影响 球蛋白浓度在五种自动化的非同位素方法上的测定 测量FT4(在ES3OO上的酶[一步法]，层I和层II[一步法] A-Half-Step]、Delfia(两步)和IMX[两步])。我们观察到 它们(A)几乎不受内源性血清结合的影响。 蛋白质和(B)似乎有足够的批内精密度 证明在患者样本上进行单次测量是合理的。 其他分析正在进行中，以评估诊断 基于放射性标记T4或T4的FT4方法的性能 类似物在非甲状腺疾病患者和有甲状腺疾病的患者中 甲状腺疾病。

Recently, nonisotopic (often automated) immunoassays for measuring serum free thyroxin (FT4) have become available. Though more costly than radioimmunoassays, they are considerably more convenient. We studied the influence of endogenous album in and thyroxin-binding globulin concentration on five automated, nonisotopic methods of measuring FT4 (Enzymun on ES3OO [one-step], Stratus I and II [one-and- a-half-step], Delfia (two-step], and IMx [two-step]). We observed that they (a) are Influenced very little by endogenous serum binding proteins and (b) seem to have suffIcIent within-run precision to Justify performIng single measurements on patIent specimens. Additional analyses are In progress to assess the diagnostic performance of FT4 methods based on the use of radiolabeled T4 or T4 analogs in patients with non-thyroidal illnesses and in those with thyroid disease.