EVALUATION OF FREE THYROXIN TECHNIQUES

游离甲状腺素技术的评估

• 批准号: 3752259
• 负责人: M ZWEIG
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3752259
• 关键词: albumins; biomedical automation; blood chemistry; clinical chemistry; diagnosis design /evaluation; endocrine disorder diagnosis; globulins; immunologic assay /test; measurement; radiotracer; serology /serodiagnosis; thyroid function; thyroid hormone binding protein; thyroxine

Recently, nonisotopic (often automated) immunoassays for measuring serum free thyroxin (FT4) have become available. Though more costly than radioimmunoassays, they are considerably more convenient. We studied the influence of endogenous album in and thyroxin-binding globulin concentration on five automated, nonisotopic methods of measuring FT4 (Enzymun on ES3OO [one-step], Stratus I and II [one-and- a-half-step], Delfia (two-step], and IMx [two-step]). We observed that they (a) are Influenced very little by endogenous serum binding proteins and (b) seem to have suffIcIent within-run precision to Justify performIng single measurements on patIent specimens. Additional analyses are In progress to assess the diagnostic performance of FT4 methods based on the use of radiolabeled T4 or T4 analogs in patients with non-thyroidal illnesses and in those with thyroid disease.

最近，用于测量的非异位（通常是自动化的）免疫测定 无血清甲状腺素（FT4）已可用。虽然更昂贵 除了放射免疫测定时，它们更方便。我们 研究了内源专辑的影响和甲状腺素结合 五个自动化的非各种方法的球蛋白浓度的浓度 测量FT4（ES3OO上的酶[一步]，I和II [一个和II A-Half步骤]，Delfia（两步]和IMX [两步]）。我们观察到了 它们（a）受内源血清结合的影响很小 蛋白质和（b）似乎具有足够的精度 证明对患者标本进行单一测量的合理性。 正在进行其他分析以评估诊断 基于使用放射性标记的T4或T4的FT4方法的性能 非甲状腺疾病患者的类似物以及患有 甲状腺疾病。