EVALUATION OF FREE THYROXIN TECHNIQUES

游离甲状腺素技术的评估

• 批准号: 3752259
• 负责人: M ZWEIG
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3752259
• 关键词: albumins; biomedical automation; blood chemistry; clinical chemistry; diagnosis design /evaluation; endocrine disorder diagnosis; globulins; immunologic assay /test; measurement; radiotracer; serology /serodiagnosis; thyroid function; thyroid hormone binding protein; thyroxine

Recently, nonisotopic (often automated) immunoassays for measuring serum free thyroxin (FT4) have become available. Though more costly than radioimmunoassays, they are considerably more convenient. We studied the influence of endogenous album in and thyroxin-binding globulin concentration on five automated, nonisotopic methods of measuring FT4 (Enzymun on ES3OO [one-step], Stratus I and II [one-and- a-half-step], Delfia (two-step], and IMx [two-step]). We observed that they (a) are Influenced very little by endogenous serum binding proteins and (b) seem to have suffIcIent within-run precision to Justify performIng single measurements on patIent specimens. Additional analyses are In progress to assess the diagnostic performance of FT4 methods based on the use of radiolabeled T4 or T4 analogs in patients with non-thyroidal illnesses and in those with thyroid disease.

最近，用于测量的非同位素（通常是自动化的）免疫测定法， 血清游离甲状腺素（FT4）已可用。虽然更昂贵 它们比放射免疫测定法方便得多。我们 研究了内源性白蛋白和甲状腺素结合的影响 球蛋白浓度的五个自动化，非同位素方法， 测量FT4（ES3OO上的Enzymun [一步法]，Stratus I和II [一步法]） 半步]、Delfia（两步）和IMx（两步））。我们观察到 它们（a）受内源性血清结合的影响很小 蛋白质和（B）似乎具有足够的运行内精密度， 说明对患者标本进行单次测量的合理性。 正在进行其他分析，以评估诊断 基于使用放射性标记T4或T4的FT4方法的性能 类似物在非甲状腺疾病患者和 甲状腺疾病