EVALUATION OF FREE THYROXIN TECHNIQUES

游离甲状腺素技术的评估

• 批准号: 3774496
• 负责人: M ZWEIG
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774496
• 关键词: albumins; clinical chemistry; diagnosis design /evaluation; diagnosis quality /standard; endocrine disorder diagnosis; immunologic assay /test; serum; thyroid function; thyroid hormone binding protein; thyroxine

Recently, nonisotopic (often automated) immunoassays for measuring serum free thyroxin (FT4) have become available. Though more costly than radioimmunoassays, they are considerably more convenient. We studied the influence of endogenous albumin and thyroxin-binding globulin concentration on five automated, nonisotopic methods of measuring FT4 (Enzymun on ES300 [one-step], Stratus I and II [one-and-a-half-step], Delfia [two-step], and IMx [two-step]). We observed that they (a) are influenced very little by endogenous serum binding proteins and (b) seem to have sufficient within-run precision to justify performing single measurements on patient specimens. Additional studies are in progress to assesses the diagnostic performance of FT4 methods based on the use of radiolabeled T4 or T4 analogs in patients with non-thyroidal illnesses and in those with thyroid disease.

最近，用于测量血清的非同位素（通常是自动化的）免疫测定法， 游离甲状腺素（FT4）已成为可用。 虽然成本高于 放射免疫测定，它们是相当方便。 我们研究了 内源性白蛋白和甲状腺素结合球蛋白影响 集中在五种自动化、非同位素测量FT 4的方法上 （ES300上的Enzymun [一步]，Stratus I和II [一步半]， 两个人，两个人，两个人，两个人。 我们观察到，（a） 受内源性血清结合蛋白的影响很小，（B）sccm 具有足够的运行内精密度，以证明进行单次 对患者标本进行测量。 其他研究正在进行中 评估FT4方法的诊断性能， 非甲状腺疾病患者中放射性标记的T4或T4类似物 以及甲状腺疾病患者。