EVALUATION OF FREE THYROXIN TECHNIQUES

最近，用于测量血清的非同位素(通常是自动化的)免疫分析 免费的甲状腺素(FT4)已经可用。尽管那时的成本更高 放射免疫分析，它们要方便得多。我们研究了 内源性白蛋白和甲状腺激素结合球蛋白浓度的影响 测定FT4(ES300上的酶)的五种自动化非同位素方法 [一步]，层一和二[一步半]，德尔菲亚[两步]，以及 IMX[两步法])。我们观察到，他们(A)受到的影响很小 内源性血清结合蛋白和(B)似乎有足够的内- 运行精确度以证明对患者执行单次测量是合理的 标本。

Recently, nonisotopic (often automated) immunoassays for measuring serum free thyroxin (FT4) have become available. Though more costly then radioimmunoassays, they are considerably more convenient. We studied the influence of endogenous albumin and thyroxin-binding globulin concentration on five automated, nonisotopic methods of measuring FT4 (Enzymun on ES300 [one-step], Stratus I and II [one-and-a-half-step], Delfia [two-step], and IMx [two-step]). We observed that they (a) are influenced very little by endogenous serum binding proteins and (b) seem to have sufficient within- run precision to justify performing single measurements on patient specimens.