EVALUATION OF FREE THYROXIN TECHNIQUES

游离甲状腺素技术的评估

• 批准号: 3837986
• 负责人: M ZWEIG
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3837986
• 关键词: albumins; clinical chemistry; diagnosis quality /standard; radioimmunoassay; radiotracer; serum; thyroid hormone binding protein; thyroxine

Recently, nonisotopic (often automated) immunoassays for measuring serum free thyroxin (FT4) have become available. Though more costly then radioimmunoassays, they are considerably more convenient. We studied the influence of endogenous albumin and thyroxin-binding globulin concentration on five automated, nonisotopic methods of measuring FT4 (Enzymun on ES300 [one-step], Stratus I and II [one-and-a-half-step], Delfia [two-step], and IMx [two-step]). We observed that they (a) are influenced very little by endogenous serum binding proteins and (b) seem to have sufficient within-run precision to justify performing single measurements on patient specimens. Additional studies are now in progress to assess the diagnostic performance of FT4 methods based on the use of radiolabeled T4 or T4 analogs in patients with non-thyroidal illnesses.

最近，用于测量血清的非同位素（通常是自动化）免疫分析 游离甲状腺素（FT4）已经可用。 虽然那时候成本更高 放射免疫测定法，它们要方便得多。 我们研究了 内源性白蛋白和甲状腺素结合球蛋白的影响 专注于五种自动、非同位素测量 FT4 的方法 （ES300 上的 Enzymun [一步]、Stratus I 和 II [一步半]、 Delfia [两步] 和 IMx [两步]）。 我们观察到它们 (a) 是 受内源血清结合蛋白的影响很小，并且（b）似乎 具有足够的运行内精度来证明执行单个 对患者样本的测量。 其他研究正在进行中 基于使用的 FT4 方法的诊断性能评估 放射性标记的 T4 或 T4 类似物用于非甲状腺疾病患者。