SOLUTE AND WATER TRANSPORT IN RENAL EPITHELIA

肾脏包含几个不同的上皮细胞，它们聚集在一起 功能，负责尿液的形成和维护 体液和电解质的动态平衡。我们正在研究这些因素的作用 上皮细胞对水、尿素、钠和尿液排泄的调节 氯化物。中心方法是从上皮细胞中分离上皮细胞 并对其体外功能进行研究。这些研究衡量了 由上皮细胞转运，表达水平不同的各种转运 肾小管中的蛋白质、单个肾脏的细胞内信号 小管段，以及所涉及的核酸(MRNA)水平 在调节细胞的运输和代谢功能方面 上皮细胞。使用数学方法对数据进行分析和整合 肾脏的转运模型。 当前的重点是： 1)加压素在水通道和尿素调节中的作用 肾集合管的载体功能； 2)尿浓缩机制的分子生理学。

The kidney contains several distinct epithelia that, in their aggregate function, are responsible for formation of the urine and the maintenance of fluid and electrolyte homeostasis. We are studying the roles of these epithelia in the regulation of the excretion of water, urea, sodium, and chloride. The central approach is to dissect the epithelia from the kidney and to study their functions in vitro. These studies measure the transport by the epithelia, the level of expression of various transport proteins in renal tubules, intracellular signalling in individual renal tubule segments, and the levels of nucleic acids (mRNA) that are involved in the regulation of both the transport and metabolic functions of the epithelia. The data are analyzed and integrated using mathematical models of transport in the kidney. The current focus is on: 1) the action of vasopressin in the regulation of water channel and urea carrier function in the renal collecting duct; 2) the molecular physiology of the urinary concentrating mechanism.