SOLUTE AND WATER TRANSPORT IN RENAL EPITHELIA

肾脏含有几种不同的上皮细胞， 功能，负责尿液的形成和维护 体液和电解质的平衡 我们正在研究这些 上皮细胞在调节水、尿素、钠和 氯化 主要的方法是将上皮细胞从 肾脏，并在体外研究其功能。 这些研究测量了 上皮细胞的转运，各种转运的表达水平 肾小管中的蛋白质，个体肾组织中的细胞内信号传导 小管节段，以及参与的核酸（mRNA）水平 在运输和代谢功能的调节中， 上皮细胞 使用数学方法对数据进行分析和整合 肾脏中的运输模型。 目前的重点是： 1)加压素在水通道和尿素调节中的作用 肾集合管中的载体功能; 2)尿液浓缩机制的分子生理学。

The kidney contains several distinct epithelia that, in their aggregate function, are responsible for formation of the urine and the maintenance of fluid and electrolyte homeostasis. We are studying the roles of these epithelia in the regulation of the excretion of water, urea, sodium, and chloride. The central approach is to dissect the epithelia from the kidney and to study their functions in vitro. These studies measure the transport by the epithelia, the level of expression of various transport proteins in renal tubules, intracellular signalling in individual renal tubule segments, and the levels of nucleic acids (mRNA) that are involved in the regulation of both the transport and metabolic functions of the epithelia. The data are analyzed and integrated using mathematical models of transport in the kidney. The current focus is on: 1) the action of vasopressin in the regulation of water channel and urea carrier function in the renal collecting duct; 2) the molecular physiology of the urinary concentrating mechanism.