SOLUTE AND WATER TRANSPORT IN RENAL EPITHELIA

肾脏包含几个不同的上皮细胞，它们聚集在一起 功能，负责尿液的形成和维护 体液和电解质的动态平衡。我们正在研究这些因素的作用 上皮细胞在调节水、尿素、氨的排泄， 小苏打、钠、钾和氯化物。一般的方法是 解剖大鼠肾脏上皮细胞并研究其功能 体外培养。这些研究测量了上皮细胞的运输功能， 催化关键化学物质的各种酶的活性 反应，以及参与其中的核酸(MRNA)的水平 对细胞的运输和代谢功能的调节 上皮细胞。使用数学方法对数据进行分析和整合 肾脏的转运模型。

The kidney contains several distinct epithelia that, in their aggregate function, are responsible for formation of the urine and the maintenance of fluid and electrolyte homeostasis. We are studying the roles of these epithelia in the regulation of the excretion of water, urea, ammonium, bicarbonate, sodium, potassium, and chloride. The general approach is to dissect the epithelia from the kidney and to study their functions in vitro. These studies measure the transport function of the epithelia, the activities of various enzymes which catalyze critical chemical reactions, and the levels of nucleic acids (mRNA) that are involved in the regulation of both the transport and metabolic functions of the epithelia. The data are analyzed and integrated using mathematical models of transport in the kidney.