SOLUTE AND WATER TRANSPORT IN RENAL EPITHELIA

肾上皮中的溶质和水运输

• 批准号: 3843288
• 负责人: M A KNEPPER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3843288
• 关键词: ammonia; bicarbonates; biological fluid transport; cell water; chlorine; electrolyte balance; enzyme activity; epithelium; ion transport; kidney cell; kidney function; kidney metabolism; mathematical model; messenger RNA; potassium; renal tubular transport; saluresis; sodium; urea

The kidney contains several distinct epithelia that, in their aggregate function, are responsible for formation of the urine and the maintenance of fluid and electrolyte homeostasis. We are studying the roles of these epithelia in the regulation of the excretion of water, urea, ammonium, bicarbonate, sodium, potassium, and chloride. The general approach is to dissect the epithelia from the kidney and to study their functions in vitro. These studies measure the transport function of the epithelia, the activities of various enzymes which catalyze critical chemical reactions, and the levels of nucleic acids (mRNA) that are involved in the regulation of both the transport and metabolic functions of the epithelia. The data are analyzed and integrated using mathematical models of transport in the kidney.

肾脏含有几种不同的上皮细胞， 功能，负责尿液的形成和维护 体液和电解质的平衡 我们正在研究这些 上皮细胞在调节水，尿素，铵， 碳酸氢盐、钠、钾和氯化物。 一般的做法是 解剖肾脏上皮细胞，研究它们在肾脏中的功能。 体外 这些研究测量上皮细胞的转运功能， 催化关键化学反应的各种酶的活性， 以及参与调控的核酸（mRNA）水平 上皮细胞的运输和代谢功能。 数据 的数学模型进行了分析和整合， 肾