PRODUCTION OF IGE RECEPTOR-DEFICIENT MICE BY HOMOLOGOUS RECOMBINATION

通过同源重组生产 IGE 受体缺陷型小鼠

• 批准号: 3768900
• 负责人: J P KINET
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3768900
• 关键词: antibody receptor; embryonic stem cell; genetic recombination; genetically modified animals; homozygote; hypersensitivity; immunoglobulin E; immunoglobulin genes; laboratory mouse; molecular cloning; receptor expression

We are attempting to generate mice lacking functional high affinity IgE receptor genes. These mice will help us to characterize the physiological function of the receptor and to further study the role of the receptor in allergy. We have cloned the alpha and beta chain genes from embryonic stem cells and have generated constructs which will be used to target the relevant genes by homologous recombination. When this is accomplished, we will attempt to produce mice in which the alpha or the beta have been knocked- out. If successful, we will be able to generate homozygotic mice in which both alleles will lack the functional genes encoding the alpha or beta gene.

我们正在尝试培育缺乏功能性高亲和力 IgE 的小鼠 受体基因。 这些小鼠将帮助我们表征 受体的生理功能并进一步研究其作用 过敏中的受体。 我们从胚胎干细胞中克隆了α链和β链基因 并生成了将用于针对相关目标的构造 基因通过同源重组。 当这一切完成后，我们将 尝试培育出α或β基因被敲除的小鼠—— 出去。 如果成功的话，我们将能够在 这两个等位基因都缺乏编码α或 β基因。