DISTRIBUTION AND ROLE OF FC EPSILON RI IN HUMAN CELLS

FC EPSILON RI 在人体细胞中的分布和作用

• 批准号: 3746643
• 负责人: J P KINET
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3746643
• 关键词: Langerhans' cell; antibody receptor; atopic dermatitis; genetically modified animals; human tissue; immunoglobulin E; laboratory mouse; monocyte; receptor expression; skin

In rodents, FcepsilonRI has only been detected so far on the surface of mast cells, basophils, and non-B non-T cells. The role played by this receptor in initiating cell degranulation and the release of the mediators of allergic reactions is clearly established. In humans however, the cell distribution of Fc(epsilon)RI appears to be different. We found last year that human Langerhans cells from the skin of normal individuals also express Fc(epsilon)RI. More recently, we also found that dermal dendritic cells express Fc(epsilon)RI as well. In addition, monocytes of allergic individuals also express this receptor. We are attempting to define the factors which regulate expression of Fc(epsilon)RI on these cells and the role of this receptor in this new context. As a way to further our understanding of these questions, we are also generating transgenic mice overexpressing human alpha chains under the control of the human promotor.

在啮齿动物中，到目前为止只在表面检测到FcepsilonRI 肥大细胞、嗜碱性粒细胞和非B类非T细胞。它所扮演的角色是 受体在启动细胞脱颗粒和释放 过敏反应的中介物已明确确立。 然而，在人类中，Fc(Epsilon)RI的细胞分布似乎是 不一样。我们去年发现人类皮肤中的朗格汉斯细胞 正常人也表达Fc(Epsilon)RI。最近，我们还 发现真皮树突状细胞也表达Fc(Epsilon)RI。在……里面 此外，过敏者的单核细胞也表达这种受体。 我们正在尝试定义调控基因表达的因素 这些细胞上的Fc(Epsilon)RI以及该受体在这一新的 背景。为了加深我们对这些问题的理解，我们 也产生了过量表达人类阿尔法链的转基因小鼠 对人类推动者的控制。