DISTRIBUTION OF FC EPSILON RI IN HUMAN CELLS

FC EPSILON RI 在人体细胞中的分布

• 批准号: 3790897
• 负责人: J P KINET
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3790897
• 关键词: Langerhans' cell; antibody receptor; atopic dermatitis; eosinophil; human tissue; immunoglobulin E; macrophage; monoclonal antibody; natural killer cells; receptor expression; skin

We have generated a monoclonal directed against the alpha chain of the human FcepsilonRI. With this reagent we found that FcepsilonRI is expressed on the surface of Langerhans cells in human skin and we are attempting to determine the possible role of this receptor in atopic dermatitis. We are also looking actively at the possibility that FcepsilonRI might be expressed in other cells such as eosinophils, macrophages, NK cells etc... Z01AI00665 The high affinity IgE receptor belongs to a class of receptors which lack intrinsic kinase activity, but activates non-receptor tyrosine and serine / threonine kinases. Receptor engagement triggers a chain of signalling events leading from protein phosphorylation to activation of phosphotidylinositol-specific phospholipase C, an increase in intracellular calcium levels, and ultimately the activation of more specialized functions. We have investigated the role of the ~ and gamma chains in signal transduction. We found (1) that receptor engagement induces immediate phosphorylation of beta (tyrosine and serine) and of gamma (tyrosine and threonine); (2) that this phosphorylation is specific to activated receptors and is reversible upon receptor disengagement; (3) that the gamma chains are critical for signal transduction but that the ~ chain does not seem necessary; (4) that phosphorylation / dephosphorylation is a coupling / uncoupling mechanism; (5) that the receptor activates two distinct phosphorylation pathways, one cell- specific and the other not. We are at present trying to identify the role of beta and are studying further the role of receptor phosphorylation in cell activation.

我们已经产生了一个单克隆针对α链的 人FcepsilonRI. 使用该试剂，我们发现FcepsilonRI是 在人类皮肤的朗格汉斯细胞表面表达， 试图确定这种受体在特应性疾病中的可能作用， 皮炎 我们也在积极研究FcepsilonRI可能成为 在其他细胞如嗜酸性粒细胞、巨噬细胞、NK细胞等中表达。 Z01AI00665 高亲和力IgE受体属于一类受体， 固有激酶活性，但激活非受体酪氨酸和丝氨酸 /苏氨酸激酶。 受体结合触发了一系列信号传导 从蛋白质磷酸化到激活 磷脂酰肌醇特异性磷脂酶C， 细胞内钙水平，并最终激活更多的 专门功能。 我们研究了~和γ的作用 信号转导链。 我们发现（1）受体结合 诱导β（酪氨酸和丝氨酸）和 γ（酪氨酸和苏氨酸）;（2）这种磷酸化是特异性的 与激活的受体结合，并且在受体脱离后可逆;（3） γ链对信号传导至关重要， 链似乎没有必要;（4）磷酸化/ 去磷酸化是偶联/解偶联机制;（5） 受体激活两个不同的磷酸化途径，一个细胞- 一个是具体的，另一个不是。 我们目前正在努力确定 并正在进一步研究受体磷酸化在 细胞激活