FOLDING ASSEMBLY, AND TRANSPORT OF VIRAL GLYCOPROTEINS

现有抗病毒疫苗的改进和 新型疫苗取决于我们对病毒的了解 附着和融合糖蛋白。 批判性地洞察 理解糖蛋白的抗原结构是由以下提供的： 研究它们与单克隆抗体（mAb）的相互作用。 用于 多年来，我们一直在研究流感病毒血凝素（HA） 糖蛋白 这种蛋白质作为其他蛋白质的模型， 类似的功能（例如HIV gp160），而且， 实际上，流感本身就是一种疾病，因为流感仍然是导致 发病率和死亡率，国家和国际。 像许多病毒糖蛋白一样，HA是一种同源寡聚体，由以下组成： 三个相同的单体亚单位。 过去一年，我们继续 研究新合成的HA的三聚化位点。 我们 以前发表的研究结果表明，HA三聚体发生在 只有在单体从ER输出之后。 了这一结论 主要基于使用标准进行的本地化研究 免疫荧光显微镜 在过去的一年里，我们增加了 使用激光共聚焦扫描显微镜的检测分辨率 来检测荧光标记的抗体，以及电子显微镜来检测 胶体金标记抗体。

The improvement of current anti-viral vaccines and the development of novel vaccines depends on increasing our understanding of viral attachment and fusion glycoproteins. Critical insight into understanding the antigenic structure of glycoproteins is provided by studying their interaction with monoclonal antibodies (mAbs). For a number of years we have studied the influenza virus hemagglutinin (HA) glycoprotein. This protein serves as a model for other proteins with similar functions (e.g. HIV gp160), and moreover, is important practically in its own right, as influenza still is a major cause of morbidity and mortality nationally, and internationally. Like many viral glycoproteins the HA is a homo-oligomer, consisting of three identical monomeric subunits. In the past year we continued to investigate the site of trimerization of newly synthesized HA. Our previously published findings suggested that HA trimerization occurs only after monomers are exported from the ER. This conclusion was based largely on localization studies performed with a standard immunofluorescence microscope. In the past year we have increased the resolution of detection by using a laser confocal scanning microscope to detect fluor-tagged antibodies, and an electron microscope to detect colloidal gold tagged antibodies.