PROCESSING OF VIRAL PROTEINS FOR T CELL RECOGNITION

用于 T 细胞识别的病毒蛋白加工

• 批准号: 6160636
• 负责人: J W YEWDELL
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6160636
• 关键词: MHC class I antigen; antigen presentation; antigenic peptide transporter; cytotoxic T lymphocyte; endopeptidases; endoplasmic reticulum; fluorescent dye /probe; immunofluorescence technique; interferon gamma; intracellular transport; laboratory mouse; oligosaccharides; peptide chemical synthesis; protein transport; proteolysis; tissue /cell culture; virus antigen; virus protein

Class I molecules of the major histocompatibility complex (MHC) bind antigens and present them to T cells bearing CD8 molecules (T/CD8+ ). T/CD8+ play a critical role in eradicating intracellular pathogens (particularly viruses) and tumors. They can also contribute to immunopathology, being involved in organ rejection and autoimmune diseases. There has been rapid progress in understanding the physical nature of the antigen-class I complex, and in how antigens are generated and become associated with class I molecules in cells. Peptides of 8 to 15 residues produced from a cytosolic pool of proteins by cytosolic proteases are translocated into the endoplasmic reticulum (ER) by a MHC encoded transporter complex known as TAP. Once in the ER, peptides (possibly after further trimming) bind to class I molecules associated with TAP and are transported to the cell surface. In this project we investigate how peptides are generated, delivered and assembled with MHC class I molecules.

主要组织相容性复合体（MHC）的I类分子结合 抗原并将其呈递给携带CD 8分子的T细胞（T/CD 8+）。 T/CD 8+在消灭细胞内病原体中发挥关键作用 （特别是病毒）和肿瘤。 他们还可以帮助 免疫病理学，参与器官排斥和自身免疫 疾病 人们对物理的理解有了快速的进步， 抗原I类复合物的性质，以及抗原是如何产生的 并与细胞中的I类分子结合。 肽8 至15个残基由细胞溶质蛋白质池产生， 蛋白酶通过MHC转位到内质网（ER）中 编码的转运蛋白复合体称为TAP。 一旦进入急诊室， （可能在进一步修剪后）与I类分子结合， 与TAP结合并被转运到细胞表面。 在这个项目中，我们 研究肽是如何产生、传递和与MHC组装的 I类分子。