PROCESSING OF VIRAL PROTEINS FOR T CELL RECOGNITION

主要组织相容性复合体(MHC)的I类分子结合 抗原并将它们呈递给带有CD8分子(T/CD8+)的T细胞。 T/CD8+在根除细胞内病原体中的关键作用 (尤其是病毒)和肿瘤。他们还可以为 参与器官排斥和自身免疫的免疫病理学 疾病。在理解物理方面有了很大的进步 I类抗原复合体的性质，以及抗原是如何产生的 并与细胞中的I类分子相关联。8的多肽 从胞浆蛋白质池中产生的15个残基 蛋白酶通过MHC转运到内质网(ER) 被称为TAP的编码传输复合体。一旦进入急诊室，多肽 (可能在进一步修剪之后)与关联的I类分子结合 并被输送到细胞表面。在这个项目中，我们 研究多肽是如何产生、传递和与MHC组装的 I类分子。

Class I molecules of the major histocompatibility complex (MHC) bind antigens and present them to T cells bearing CD8 molecules (T/CD8+ ). T/CD8+ play a critical role in eradicating intracellular pathogens (particularly viruses) and tumors. They can also contribute to immunopathology, being involved in organ rejection and autoimmune diseases. There has been rapid progress in understanding the physical nature of the antigen-class I complex, and in how antigens are generated and become associated with class I molecules in cells. Peptides of 8 to 15 residues produced from a cytosolic pool of proteins by cytosolic proteases are translocated into the endoplasmic reticulum (ER) by a MHC encoded transporter complex known as TAP. Once in the ER, peptides (possibly after further trimming) bind to class I molecules associated with TAP and are transported to the cell surface. In this project we investigate how peptides are generated, delivered and assembled with MHC class I molecules.