INTRACELLULAR ANTIBODY-MEDIATED VIRUS NEUTRALIZATION

许多对病毒核心蛋白或非结构蛋白特异的抗体 有能力阻止病毒感染，如果他们被引入到 病毒感染细胞的胞质溶胶。 抗体认识的新进展 折叠表明抗体具有适当折叠和结合的能力 当在胞质溶胶中表达时， 因此，细胞或转基因 表达细胞溶质形式的抗体的动物 应该能抵抗病毒感染。 使用 电穿孔以诱导抗体进入我们已经鉴定的细胞 单克隆抗体阻断流感病毒感染， 开始从杂交瘤细胞中克隆相应的基因， 创造出能抵抗流感病毒感染的动物。

Many antibodies specific for viral core proteins or non-structural proteins have the capacity to block viral infection if they are introduced into the cytosol of virus infected cells. Recent advance in understanding antibody folding indicate that antibodies have the ability to properly fold and bind antigen when expressed in the cytosol. Therefore, cells, or transgenic animals expressing a cytosolic form of an antibody capable of interfering with viral replication should be resistant to viral infection. Using electroporation to induce antibodies into cells we have identified monoclonal antibodies that block infection with influenza viruses, and have begun to clone the corresponding genes from hybridoma cells with the goal of creating animals resistant to influenza virus infection.