APPLICATIONS OF NEW MASS SPECTRAL TECHNIQUES

该项目的目标是研究和开发新的质量 光谱技术，以便提供创新的和/或更快速的 解决问题，包括（1）化学结构测定，（2） 复杂混合物分析和（3）痕量组分的测量 生物系统。 快原子轰击质谱和 液相色谱-质谱法仍然是目前的技术 兴趣 反相，成对离子技术正在开发， 微柱液相色谱与连续流动快速 在线液相色谱-质谱联用的原子轰击电离 核苷的光谱分析。 使用酸性阴离子表面活性剂 在静态快速原子轰击过程中， 胞嘧啶核苷如环戊烯基胞嘧啶的分析。 的 苯-3-甲酰胺核苷体外代谢产物结构， 是一种有效的肌苷一磷酸脱氢酶抑制剂， 经负离子快速测定，证实为腺嘌呤二核苷酸结合物 原子轰击质谱法

The objective of this project is research on and development of new mass spectral techniques in order to provide innovative and/or more rapid solutions to problems involving (1) chemical structure determination, (2) complex mixture analysis and (3) measurement of trace components in biological systems. Fast atom bombardment mass spectrometry and combined liquid chromatography-mass spectrometry remain the techniques of current interest. Reversed phase, paired-ion techniques are being developed for combining micro-column liquid chromatography with continuous-flow fast atom bombardment ionization for the on-line liquid chromatography-mass spectrometry analysis of nucleosides. Use of acidic, anionic surfactants during static fast atom bombardment greatly enhances sensitivity for analysis of cytosine nucleosides such as cyclopentenyl cytosine. The structure of an in vitro metabolite of benzene-3-carboxamide riboside, which is a potent inhibitor of inosine monophosphate dehydrogenase, has been confirmed as the adenine dinucleotide conjugate by negative ion fast atom bombardment mass spectrometry.