BIOLOGY OF BRAIN TUMORS

加州大学洛杉矶分校脑肿瘤研究中心的使命是建立一个 多学科的学术努力，研究脑肿瘤， 新的生物治疗方法。 该组人员包括 神经外科，神经病学，分子生物学， 免疫学和生物技术。 该中心的主题是生物 星形细胞脑肿瘤在分子水平上的特征。 新 脑肿瘤生物学知识将导致该计划的新的临床 接近。 特别是基因、受体和发育 脑肿瘤特有的调节蛋白将被利用， 反义和其他生物学方法。 一系列高度集中的多学科研究项目将在 在分子生物学、免疫学和生物技术领域开展研究。 的 该项目将得到脑组织/临床数据库的支持。 项目 #1将制备实体瘤和细胞系的分子图谱 （主要通过北方印迹分析）使用=癌基因和编码 发育调节的中间丝蛋白 标记和重要受体。 这一做法将得到以下方面的补充： 利用消减杂交技术寻找新的标记。 Project #2 将集中在离体肿瘤和早期传代中的基因表达 cultures. 项目#3基于令人兴奋的初步数据， 外周苯二氮卓受体反义寡核苷酸 将阐明基因的表达/缺失如何影响肿瘤细胞 生存 对新发现的基因也将进行类似的分析 从项目#1中减去杂交。 项目#4将研究 免疫-肿瘤细胞网络。 细胞炎性反应的特点 表型和细胞因子反应将被定义， 将检查肿瘤和炎症反应之间的作用。 项目#5将开发并将其应用于临床实践技术， 将在手术中识别大脑和肿瘤的功能区域。 我们 将通过以下方式完成我们的使命： - 建立和维持一个组织结构， 促进和加强多学科、跨部门和 促进研究、传播研究成果和培训的机构间努力 脑肿瘤专家 - 评估中心的进展，并开始确定和吸引其他 资金资源，以确保加州大学洛杉矶分校脑肿瘤的活动， 研究中心可以扩大超过三年的资金， 可行性补助金。

The mission of the UCLA Brain Tumor Research Center is to establish a multidisciplinary academic effort to study brain tumors and to develop new biologically based treatment approaches. The team consists of researchers and clinicians in neurosurgery, neurology, molecular biology, immunology and biotechnology. The theme of the Center is the biological characterization of astrocytic brain tumors at the molecular level. New knowledge of brain tumor biology will lead this program to novel clinical approaches. In particular, genes, receptors, and developmentally regulated proteins unique to brain tumors will be exploited using antisense and other biological approaches. A series of highly focused multidisciplinary research projects will be conducted in molecular biology, immunology and biotechnology. The project will be supported by a Brain Tissue/Clinical Data Bank. Project #1 will prepare molecular profiles of solid tumors and cell lines (primarily by Northern blot analysis) using =oncogenes and genes coding for intermediate filament proteins developmentally regulated neural markers, and important receptors. This approach will be complemented by subractive hybridization technology to find novel markers. Project #2 will focus on gene expression in both the ex vivo tumor and early passage cultures. Project #3 building on exciting preliminary data using antisense oligodeoxynucleotides to the peripheral benzodiazepine receptor will elucidate how the expression/absence of genes influences tumor cell survival. Similar analyses will be carried out for new genes identified by substractive hybridization from project #1. Project #4 will study immune-tumor cell networks. The character of the cellular inflammatory phenotype and cytokine response will be defined and mutual interactive effects between the tumor and inflammatory response will be examined. Project #5 will develop and bring to clinical practice technology which will identify functional areas of brain and tumor intraoperatively. We will accomplish our mission by: -Establishing and maintaining an organizational structure which will facilitate and enhance multidisciplinary, interdepartmental, and interagency efforts to promote research, disseminate findings, and train specialists in brain tumors. -Evaluating Center progress and begin to identify and attract other funding resources to assure that the activities of the UCLA Brain Tumor Research Center can expand beyond the three-year funding of the feasibility grant.