SEROLOGICAL RESPONSE TO BORDETELLA PERTUSSIS ANTIGENS

对百日咳博德特氏菌抗原的血清学反应

• 批准号: 3792359
• 负责人: D L BURNS
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792359
• 关键词: Bordetella pertussis; active immunization; adenylate cyclase; antibacterial antibody; bacterial antigens; bactericidal immunity; cellular immunity; child (0-11); hemolysin; human tissue; humoral immunity; immunoglobulin G; infant human (0-1 year); molecular chaperones; oligosaccharides; pertussis; pertussis toxin; pertussis vaccine; placental transfer; protein folding; serology /serodiagnosis; serum; stress proteins; western blottings

The human serological response to several envelope-associated proteins and adenylate cyclase toxin of B. pertussis was examined using immunoblot techniques. Antigens recognized by sera from individuals with culture-confirmed pertussis as well as by sera from infants immunized with three doses of conventional whole-cell pertussis vaccine included a 63,000 Da protein that was shown to be antigenically related to a mycobacterial heat-shock protein and structurally related to the chaperonin family of proteins involved in protein folding and assembly. B. pertussis lipooligosaccharide was also recognized by antibodies in certain of these sera. A 29,000 Da species reacted with sera from convalescent individuals whereas a 91,000 Da species reacted with sera from vaccinated individuals. Antibodies to adenylate cyclase toxin were common in sera from individuals diagnosed with pertussis, however these antibodies were rarely found in children who had received three doses of DTP vaccine. Sera from neonates (cord blood) and corresponding maternal sera were examined for antibodies to Bordetella adenylate cyclase. Similar levels of antigen specific antibodies (IgG isotype) were found in each serum sample of a given neonate-maternal pair suggesting that antibodies to adenylate cyclase are passively transferred through the placenta. These antibodies may have been elicited by previous exposure of the mothers to B. pertussis or by vaccination of the mother with pertussis vaccine since all four pertussis vaccines currently in use in the US were capable of eliciting in mice antibodies reactive with adenylate cyclase. Alternatively, these antibodies may have been elicited by exposure of mothers to cross-reactive antigens such as E. coli a-hemolysin since all of the neonatal sera contained antibodies reactive with this antigen. Antibodies to adenylate cyclase have been previously shown to passively protect mice against challenge with B. pertussis. Therefore the possibility exists that these antibodies may help protect the newborn child against pertussis.

人对几种乳腺癌相关蛋白的血清学反应 和B的腺苷酸环化酶毒素。用免疫印迹法检测百日咳 技术.由来自患有以下疾病的个体的血清识别的抗原： 培养证实的百日咳以及来自免疫的婴儿的血清 三剂常规全细胞百日咳疫苗包括63，000 Da蛋白，该蛋白被证明是抗原相关的， 分枝杆菌热休克蛋白和结构相关的 参与蛋白质折叠和装配的蛋白质伴侣蛋白家族。 B。百日咳脂寡糖也被抗体识别， 其中的一些sera。一种29，000 Da的物质与来自 恢复期个体，而91，000 Da的物种与血清反应， 接种疫苗的人。腺苷酸环化酶毒素抗体， 常见于诊断为百日咳的个体的血清中，然而这些 在接受过三次注射的儿童中很少发现抗体， DTP疫苗 新生儿血清（脐带血）和相应的 检测孕妇血清中的腺苷酸博德特氏菌抗体 环化酶抗原特异性抗体（IgG同种型）水平相似， 在给定的产妇-产妇配对的每个血清样本中发现， 腺苷酸环化酶的抗体通过 胎盘 这些抗体可能是由先前的 母亲暴露于B。百日咳或母亲接种疫苗 由于目前使用的所有四种百日咳疫苗 在美国，能够在小鼠中引发与以下物质反应的抗体： 腺苷酸环化酶或者，这些抗体可能是由 通过将母亲暴露于交叉反应性抗原如E. 杆菌 a-溶血素，因为所有的新生儿血清都含有抗体反应 这个抗原。腺苷酸环化酶的抗体以前已经被发现。 显示被动保护小鼠免受B攻击。百日咳。 因此，这些抗体可能有助于保护 新生儿对百日咳。