SEROLOGICAL RESPONSE TO BORDETELLA PERTUSSIS ANTIGENS

对百日咳博德特氏菌抗原的血清学反应

• 批准号: 3804640
• 负责人: D L BURNS
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3804640
• 关键词: Bordetella pertussis; Mycobacterium; adenylate cyclase; antigen antibody reaction; antiserum; bacterial proteins; blood chemistry; cross immunity; diphtheria toxoid; hospital patient care; human tissue; humoral immunity; immunotherapy; infant human (0-1 year); laboratory mouse; oligosaccharides; passive immunization; pertussis; pertussis toxin; pertussis vaccine; protein folding; protein structure; protein transport; serology /serodiagnosis; stress proteins; tetanus toxoid; tissue /cell culture; western blottings

The human serological response to several envelope-associated proteins and adenylate cyclase toxin of B. pertussis was examined using immunoblot techniques. Antigens recognized by sera from individuals with culture- confirmed pertussis as well as by sera from infants immunized with three doses of conventional whole-cell pertussis vaccine included a 63,000 Da protein that was shown to be antigenically related to a mycobacterial heat- shock protein and structurally related to the chaperonin family of proteins involved in protein folding and assembly. B. pertussis lipooligosaccharide was also recognized by antibodies in certain of these sera. A 29,000 Da species reacted with sera from convalescent individuals whereas a 91,000 Da species reacted with sera from vaccinated individuals. Antibodies to adenylate cyclase toxin were common in sera from individuals diagnosed with pertussis, however these antibodies were rarely found in children who had received three doses of DTP vaccine. Sera from neonates (cord blood) and corresponding maternal sera were examined for antibodies to Bordetella adenylate cyclase. Similar levels of antigen specific antibodies (IgG isotype) were found in each serum sample of a given neonate-maternal pair suggesting that antibodies to adenylate cyclase are passively transferred through the placenta. These antibodies may have been elicited by previous exposure of the mothers to B. pertussis or by vaccination of the mother with pertussis vaccine since all four pertussis vaccines currently in use in the US were capable of eliciting in mice antibodies reactive with adenylate cyclase. Alternatively, these antibodies may have been elicited by exposure of mothers to cross-reactive antigens such as E. coli a-hemolysin since all of the neonatal sera contained antibodies reactive with this antigen. Antibodies to adenylate cyclase have been previously shown to passively protect mice against challenge with B. pertussis. Therefore the possibility exists that these antibodies may help protect the newborn child against pertussis.

人血清学反应的几种疾病相关蛋白和 B腺苷酸环化酶毒素。用免疫印迹法检测百日咳 技术. 被来自具有培养物的个体的血清识别的抗原- 证实的百日咳，以及从婴儿的血清免疫与三个 常规全细胞百日咳疫苗的剂量包括63，000 Da 蛋白质，被证明是抗原相关的分枝杆菌热- 休克蛋白和结构上与蛋白质的伴侣蛋白家族相关 参与蛋白质折叠和组装。 B。百日咳脂寡糖 也被某些血清中的抗体识别。 A 29，000 Da 91，000 Da的抗血清与恢复期个体的血清反应， 种属与接种个体的血清发生反应。 抗体 腺苷酸环化酶毒素常见于诊断为 然而，这些抗体很少在患有百日咳的儿童中发现。 接种了三剂百白破疫苗 来自新生儿的血清（脐带血）和相应的母体血清， 检测博德特氏菌腺苷酸环化酶抗体。 相似水平的 在每个血清样品中发现抗原特异性抗体（IgG同种型 表明腺苷酸抗体 环化酶通过胎盘被动转移。 这些抗体 可能是由于母亲之前接触过B。百日咳 或通过母亲接种百日咳疫苗，因为所有四种疫苗 目前在美国使用的百日咳疫苗能够引起 与腺苷酸环化酶反应的小鼠抗体。 或者，这些 抗体可能是由母亲暴露于交叉反应性 抗原如E.大肠杆菌a-溶血素，因为所有新生儿血清 含有与这种抗原反应的抗体。 腺苷酸抗体 环化酶先前已经显示出被动地保护小鼠免受 挑战B。百日咳。 因此，存在的可能性是， 抗体可能有助于保护新生儿免受百日咳的侵害。