SEROLOGICAL RESPONSE TO BORDETELLA PERTUSSIS ANTIGENS

对百日咳博德特氏菌抗原的血清学反应

• 批准号: 3811001
• 负责人: D L BURNS
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3811001
• 关键词: Bordetella pertussis; Mycobacterium; adenylate cyclase; antigen antibody reaction; antiserum; bacterial proteins; blood chemistry; diphtheria toxoid; hospital patient care; human tissue; humoral immunity; immunotherapy; infant human (0-1 year); oligosaccharides; passive immunization; pertussis; pertussis toxin; pertussis vaccine; protein folding; protein structure; protein transport; serology /serodiagnosis; stress proteins; tetanus toxoid; tissue /cell culture; western blottings

The human serological response to several envelope-associated proteins and adenylate cyclase toxin of B. pertussis was examined using immunoblot techniques. Antigens recognized by sera from individuals with culture-confirmed pertussis as well as by sera from infants immunized with three doses of conventional whole-cell pertussis vaccine included a 63,000 Da protein that was shown to be antigenically related to a mycobacterial heat-shock protein and structurally related to the chaperonin family of proteins involved in protein folding and assembly. B. pertussis lipooligosaccharide was also recognized by antibodies in certain of these sera. A 29,000 Da species reacted with sera from convalescent individuals whereas a 91,000 Da species reacted with sera from vaccinated individuals. Antibodies to adenylate cyclase toxin were common in sera from individuals diagnosed with pertussis, however these antibodies were rarely found in children who had received three doses of DTP vaccine. These data suggest that some of these antigens may play a role in immunity to pertussis and that further investigation is warranted.

人类对几种包膜相关蛋白的血清学反应 和百日咳杆菌腺苷环化酶毒素的检测。 免疫印迹技术。个体血清识别的抗原 经培养确认的百日咳以及婴儿的血清 三剂常规全细胞百日咳疫苗免疫效果观察 包括一种被证明与抗原性相关的63,000 Da蛋白质 一种分枝杆菌热休克蛋白，在结构上与 伴侣蛋白家族，参与蛋白质折叠和组装。 百日咳杆菌脂低聚糖也能被抗体识别 这些血清中的某些。29,000个Da物种与来自中国的血清反应 恢复期的个体，而91,000 Da的物种与血清发生反应 来自接种疫苗的个人。腺苷环化酶毒素的抗体是 在被诊断为百日咳的人的血清中很常见，然而这些 在接种了三剂疫苗的儿童中很少发现抗体 DTP疫苗。这些数据表明，这些抗原中的一些可能发挥着 在百日咳免疫中的作用，进一步的研究是 这是正当的。