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SECRETION OF PERTUSSIS TOXIN FROM BORDETELLA PERTUSSIS

SECRETION OF PERTUSSIS TOXIN FROM BORDETELLA PERTUSSIS
百日咳博德特氏菌分泌百日咳毒素
批准号:
2568889
负责人:
D L BURNS
金额:
$0.0万
依托单位:
--
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:

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中文摘要
翻译
之前,我们证明了一组8个基因的存在
英文摘要
Previously, we demonstrated the existence of a set of eight genes that are essential for the secretion of pertussis toxin (PT) from B. pertussis. These genes, termed ptl genes, encode transport proteins that share homology with a family of proteins involved in the transport of both DNA and proteins across bacterial membranes. We now have evidence for a ninth secretion gene. This small gene, ptl1 is predicted to encode a protein of 6.8 kDa and is located between ptlD and ptlE. PtlI shares homology with VirB7 which is required for transport of T-DNA across the membranes of the plant pathogen Agrobacterium tumefaciens. Deletion of the ptlI region from B. pertussis has no discernable effect of PtlE but resulted in loss of detectable PtlF, suggesting the PtlI may interact with and stabilize PtlF. Using immunoprecipitation techniques, we demonstrated a direct interaction between PtlI and PtlF. These results are the first evidence of interactions between Ptl proteins. In addition to the work on elucidation of interactions between Ptl proteins, we are also investigating the mechanism by which the Ptl proteins promote transport of PT. One Ptl protein in particular, PtlC, has the interesting characteristic that it contains a nucleotide binding motif. We are currently purifying this protein and determining whether this protein has either hydrolase or kinase activity that might play a critical role in the transport process. This work increases our knowledge of the secretion of PT and may aid in the construction of strains of B. pertussis which efficiently produce and secrete PT. Such strains would be useful for vaccine production. In addition, knowledge of the mechanism of PT secretion might aid in the development of live-attenuated vaccines that secrete inactivated forms of PT, thus inducing a protective immune response.
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STUDIES OF THE STRUCTURE AND FUNCTION OF PERTUSSIS TOXIN
  • 批准号:
    2568888
  • 项目类别:
  • 资助金额:
    $0.0万
  • 财政年份:
    --
  • 负责人:
    D L BURNS
  • 依托单位:
    --
MOLECULAR CHAPERONES
  • 批准号:
    3770287
  • 项目类别:
  • 资助金额:
    $0.0万
  • 财政年份:
    --
  • 负责人:
    D L BURNS
  • 依托单位:
    --
SEROLOGICAL RESPONSE TO BORDETELLA PERTUSSIS ANTIGENS
  • 批准号:
    3804640
  • 项目类别:
  • 资助金额:
    $0.0万
  • 财政年份:
    --
  • 负责人:
    D L BURNS
  • 依托单位:
    --
DEVELOPMENT OF TESTS FOR ACELLULAR PERTUSSIS VACCINES
  • 批准号:
    3804655
  • 项目类别:
  • 资助金额:
    $0.0万
  • 财政年份:
    --
  • 负责人:
    D L BURNS
  • 依托单位:
    --
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