ULTRASTRUCTURAL LOCATION OF PNEUMOLYSIN IN S. PNEUMONIAE

肺炎球菌溶血素在不同类型肺炎球菌中的细胞定位， 免疫电镜观察。 肺炎球菌19 F型细胞， 8~10型和8-CB型，以及未被囊化的Rx 1株， 用兔抗肺炎球菌溶血素血清处理，然后 用胶体金标记羊抗兔IgG。 然后将细胞 与Chrome锇固定剂反应，脱水，包埋在环氧树脂中 切片并用电子显微镜检查。 肺炎球菌溶血素的细胞定位在所有4株中相似 考察 在细胞的外部没有发现标记， 壁或胶囊;表明在这些位置没有层。 在 此外，完全完整的细胞没有结合的细胞内金颗粒， 由于抗体不能穿透致密的细胞壁 矩阵 溶血素标记与粘附于 从细胞膜延伸到细胞质。 金颗粒 在裂解细胞内的粘附结构的外围可见。 的 该物质的量似乎与测得的肺炎球菌溶血素相关 活性;显示较高肺炎球菌溶血素的Rx 1和8-10型菌株 活性，显示出比对照组更多的粘附材料和标记抗体。 8型-CB显示出低肺炎球菌溶血素活性。

Cellular localization of pneumolysin in various types of pneumococci was studied by the immunoelectron microscopy. Cells of pneumococcal type 19F, type 8~10 and 8-CB, and non-capsulated Rx1 strain were fixed in paraformaldehyde and treated with rabbit anti-pneumolysin serum, followed by goat-anti-rabbit IgG labeled with colloidal gold. The cells were then reacted with chrome osmium fixative, dehydrated, embedded in epoxy resin sectioned and examined with an electropn microscope. The cellular localization of pneumolysin was similar in all 4 strains examined. No labeling was found on the exterior of the cell, either cell wall or capsule; indicating the absence of ply in these locations. In addition, totally intact cells had no bound intracellular gold particles, due to the inability of the antibodies to penetrate the dense cell wall matrix. Pneumolysin labeling was associated with a structure adherent to the cell membrane and extending into the cytoplasm. Gold particles were seen on the periphery of the adherent structures inside lysed cells. The amount of this material seems to correlate with the measured pneumolysin activity; Rx1 and type 8-10 strains that showed higher pneumolysin activity, demonstrated more adherent material and labeled antibodies than type 8-CB that showed a low pneumolysin activity.