ISOLATION AND CHARACTERIZATION OF IMMUMODULATING PLANT P

几种草药包括（a）黄芪，（B）蛇床子， (c)据报道，当归和（d）Hoelen表现出 刺激免疫系统的活性并且相对无毒。 多糖是从这些植物药中分离出来的。 他们的 理化性质的特点，包括糖含量和 分子量 对白细胞介素（IL）-2，IL-4和 研究了用当归PS或当归多糖处理的小鼠脾细胞中的IL-6。 Hoelen PS，随后用肺炎球菌9V型PS-ply免疫 共轭 用Angelica PS或Hoelen PS和9V型PS-层处理的小鼠 缀合物产生高1.5至3.9倍的IL-2和高4.6至6.4倍的IL-2。 IL-4水平高于单独给予缀合物的组。 植物PS没有 刺激IL-6产生。 因此，这些植物PS增强了肺炎球菌 PS缀合物以产生更多的IL-2和IL-4。 抗体对 在用当归处理的成年小鼠中研究了9V型PS-ply缀合物 PS或Hoelen PS。 血清抗9V PS IgG和IgM抗体水平分别为 与未处理的小鼠相比，Angelica PS或Hoelen PS增强了 对照 这些结果表明，增强的免疫反应 植物PS诱导的肺炎球菌PS-蛋白结合物可能是由 通过细胞因子的活动。

Several herb medicines including (a) Astragali radix, (b) Cnidii rhizoma, (c) Angelicae radix, and (d) Hoelen have been reported to exhibit stimulating activities on the immune system and are relatively nontoxic. Polysaccharides were isolated from these plant medicines. Their physicochemical properties were characterized including sugar contents and molecular weights. Effects on production of interleukin (IL)-2, IL-4 and IL-6 were studied in spleen cells of mice treated with Angelica PS or Hoelen PS followed by immunization with pneumococcal type 9V PS-ply conjugate. Mice treated with Angelica PS or Hoelen PS and type 9V PS-ply conjugate produced 1.5 to 3.9 fold higher IL-2 and 4.6 to 6.4 fold higher IL-4 levels than the group given the conjugate alone. Plant PS did not stimulate IL-6 production. Thus, these plant PS enhanced the pneumococcal PS conjugate to produce more IL-2 and IL-4. The antibody response to the type 9V PS-ply conjugate was studied in adult mice treated with Angelica PS or Hoelen PS. Serum levels of anti-9V PS IgG and IgM antibodies were enhanced in the mice by Angelica PS or Hoelen PS compared to non-treated controls. These results indicate that the enhanced immune response to pneumococcal PS-protein conjugate induced by plant PS may be mediated through the activities of cytokines.