ISOLATION AND CHARACTERIZATION OF IMMUNOMODULATING PLANT POLYSACCHARIDES

几种中草药，包括(A)黄芪，(B)蛇床子， (C)当归和(D)霍伦据报道将展出 对免疫系统有刺激作用，相对无毒。 从这些植物药材中分离得到多糖。他们的 理化性质的表征包括糖含量和 分子量。对白介素2、白介素4和白介素2产生的影响 当归多糖对小鼠脾细胞IL-6的影响 霍伦PS后用肺炎球菌9V PS-PLY免疫 共轭。当归多糖和9V PS-PLY对小鼠的治疗作用 结合物产生的IL-2高1.5至3.9倍，高4.6至6.4倍 IL-4水平高于单独给予结合物组。植物PS没有 刺激IL-6的产生。因此，这些植物PS增强了肺炎球菌 PS结合产生更多的IL-2和IL-4。对该病毒的抗体反应 当归对成年小鼠的9V PS-PLY结合物的研究 PS或Hoelen PS。血清抗9V PS免疫球蛋白Ig G和Ig M抗体 当归多糖或虎杖多糖对小鼠的促进性作用 控制。这些结果表明，增强的免疫反应对 植物PS诱导的肺炎链球菌PS-蛋白结合物可能是介导性的 通过细胞因子的活动。

Several herb medicines including (a) Astragali radix, (b) Cnidii rhizoma, (c) Angelicae radix, and (d) Hoelen have been reported to exhibit stimulating activities on the immune system and are relatively nontoxic. Polysaccharides were isolated from these plant medicines. Their physicochemical properties were characterized including sugar contents and molecular weights. Effects on production of interleukin (IL)-2, IL-4 and IL-6 were studied in spleen cells of mice treated with Angelica PS or Hoelen PS followed by immunization with pneumococcal type 9V PS-ply conjugate. Mice treated with Angelica PS or Hoelen PS and type 9V PS-ply conjugate produced 1.5 to 3.9 fold higher IL-2 and 4.6 to 6.4 fold higher IL-4 levels than the group given the conjugate alone. Plant PS did not stimulate IL-6 production. Thus, these plant PS enhanced the pneumococcal PS conjugate to produce more IL-2 and IL-4. The antibody response to the type 9V PS-ply conjugate was studied in adult mice treated with Angelica PS or Hoelen PS. Serum levels of anti-9V PS IgG and IgM antibodies were enhanced in the mice by Angelica PS or Hoelen PS compared to non-treated controls. These results indicate that the enhanced immune response to pneumococcal PS-protein conjugate induced by plant PS may be mediated through the activities of cytokines.