DEFENSE AGAINST NEONATAL INFECTION VIA MATERNAL IMMUNITY

对母体免疫的影响进行了研究。 幼年小鼠对肺炎链球菌9V型的后续免疫应答 多糖(PS)、肺溶酶(PLY)和9V PS-PLY结合物。年青的 来自母亲的小鼠，免疫9V PS、灭活溶血素或 9V PS-PLY结合物在孕期接受额外剂量的 断奶时也有相同的抗原。这些小鼠产生了更高的特异性IgM抗体 而不是来自未免疫母亲的幼鼠。此外，来自中国的幼鼠 孕期接种19F PS-PLY结合物的母亲 在出生后接受额外剂量的相同结合物 未免疫幼鼠19F PS免疫球蛋白Ig G和Ig M抗体高于幼鼠 母亲们。在5 x 104挑战后快速清除细菌 在接种了19F肺炎球菌的幼鼠中观察到CFU/剂量 围产期~(19)F PS结合物与未免疫者的比较 控制。这些结果表明，母体免疫接种 孕期肺炎链球菌PS蛋白结合物及给药 出生后给幼鼠的另一种结合物具有保护作用 小鼠对肺炎球菌感染的免疫力。

Studies were conducted on the effect of maternal immunization on the subsequent immune response of young mice to pneumococcal type 9V polysaccharide (PS), pneumolysin (ply), and 9V PS-ply conjugate. Young mice from mothers immunized with type 9V PS, inactivated pneumolysin, or 9V PS-ply conjugate during pregnancy received an additional dose of the same antigen at weaning. These mice produced higher specific IgM antibody than young mice from unimmunized mothers. Furthermore, young mice from mothers immunized with type 19F PS-ply conjugate during pregnancy that received an additional dose of the same conjugate after birth produced higher 19F PS IgG and IgM antibodies than young mice from unimmunized mothers. Rapid bacterial clearance following challenge with 5 x 104 cfu/dose of 19F pneumococci was observed in young mice that had received 19F PS conjugate during perinatal period as compared to the non-immunized controls. These results indicate that maternal immunization with pneumococcal PS-protein conjugate during pregnancy and administration of an additional conjugate to young mice after birth conferred protective immunity against pneumococcal infection in mice.