ACQUISITION OF AN INCREASED RESISTANCE TO HOST DEFENSES IN VIVO

体内获得增强的宿主防御抵抗力

• 批准号: 3790851
• 负责人: T A RUSSO
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3790851
• 关键词: Escherichia coli; bacterial cytopathogenic effect; gene induction /repression; genetic library; genetic transcription; hemoglobin; host organism interaction; immunity; phenotype; regulatory gene; serum

Microbes are versatile organisms that have the ability to adapt to a myriad of environments in which they must subsist. Pathogenic bacteria, such as Escherichia coli are no exception to this rule. Its phenotypic characteristics in in vitro medium such as LB broth are not equivalent to its phenotype when it causes a bacteremia. These changes in vivo undoubtedly play an important role in pathogenesis. We are therefore interested in identifying the genes responsible for these in vivo phenotypic changes and their mode of induction. Initial studies suggest that the combination of serum plus hemoglobin can induce these gene products. To identify these genes we will use either transcriptional fusions or create a subtraction library. Once the genes of interest are identified their protein products can be elucidated.

微生物是多功能的生物体，它们有能力适应环境。 它们必须生存的无数环境。 致病菌， 如大肠杆菌也不例外。 其表型 在体外培养基（如LB肉汤）中的特性不等同 当它引起菌血症时，它的表型。 这些变化在体内 无疑在发病机制中起重要作用。 因此我们 有兴趣在体内鉴定这些基因， 表型变化及其诱导模式。 初步研究表明 血清和血红蛋白的组合可以诱导这些基因 产品. 为了识别这些基因，我们将使用转录 融合或创建消减文库。 一旦感兴趣的基因 鉴定了它们的蛋白质产物。