IDENTIFICATION OF COMPLEMENT RESISTANCE DETERMINANTS IN ESCHERICHIA COLI

大肠杆菌中补体抗性决定因素的鉴定

• 批准号: 3809751
• 负责人: T A RUSSO
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3809751
• 关键词: Escherichia coli; bacterial genetics; complement; gene expression; genetic manipulation; mutant; transposon /insertion element; virulence

Complement resistance is an important virulence factor in the pathogenesis of bacterial gram negative infections. A more complete understanding of its complement resistance determinants may lead to improved or novel modalities for therapeutic intervention as well as an insight as to the mechanism of complement's action.The experimental approach taken for this project involves transposon mutagenesis of the chromosome and plasmid(s) of a clinical isolate of Escherichia coli that is highly complement resistant. We are using a transposon system (TnphoA) designed to mutagenize and identify mutants in outer, periplasmic and cytoplasmic membrane proteins. Mutants are screened for an increased sensitivity to serum and the acquisition of this phenotype will presumably be the result of the disruption of genes that play a role in complement resistance.This method makes no assumptions as to what genes contribute to complement resistance and will hopefully identify previously unrecognized gene products that play a role in this process.

补体抵抗是其发病机制中的重要毒力因子 革兰氏阴性菌感染的可能性更全面地了解其 补体抵抗决定因素可能导致改进的或新的模式 对于治疗干预，以及洞察的机制， 补充的行动。该项目采取的实验方法 涉及转座子诱变的染色体和质粒， 一种临床分离的大肠杆菌，对补体具有高度抗性。 我们正在使用转座子系统（TnphoA），旨在诱变和 鉴定外膜、周质膜和细胞质膜蛋白的突变体。 筛选突变体以增加对血清的敏感性， 这种表型的获得可能是 破坏在补体抗性中起作用的基因。这种方法 没有假设哪些基因导致补体抵抗 并有望发现以前未被识别的基因产物， 在这个过程中的作用。