IDENTIFICATION OF COMPLEMENT RESISTANCE DETERMINANTS IN ESCHERICHIA COLI

大肠杆菌中补体抗性决定因素的鉴定

• 批准号: 3803268
• 负责人: T A RUSSO
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3803268
• 关键词: Escherichia coli; bacterial genetics; bactericidal immunity; complement inhibitors; gene expression; gram negative bacteria; membrane proteins; opsonin; transposon /insertion element; virulence

Complement resistance is an important virulence factor in the pathogenesis of bacterial gram negative infections. A more complete understanding of its complement resistance determinants may lead to improved or novel modalities for therapeutic intervention as well as an insight as to the mechanism of complement's action. The experimental approach taken for this project involves transposon mutagenesis of the chromosome and plasmid(s) of a clinical isolate of Escherichia coli that is highly complement resistant. We are using a transposon system (TnphoA) designed to mutagenize and identify mutants in outer, periplasmic and cytoplasmic membrane proteins. Mutants are screened for an increased sensitivity to serum and the acquisition of this phenotype will presumably be the result of the disruption of genes that play a role in complement resistance.This method makes no assumptions as to what genes contribute to complement resistance and will hopefully identify previously unrecognized gene products that play a role in this process.

补体抵抗是一个重要的毒力因子， 革兰氏阴性细菌感染的发病机制。 更完整 了解其补体抵抗决定因素可能会导致 用于治疗干预的改进的或新的模式以及 深入了解补体的作用机制。实验 该项目采用的方法涉及转座子诱变的 大肠杆菌临床分离株的染色体和质粒， 是高度补体抗性的。 我们使用转座子系统 （TnphoA）设计用于诱变和鉴定外， 周质膜和细胞质膜蛋白。 筛选突变体， 对血清的敏感性增加和这种表型的获得 可能是基因破坏的结果， 在补体抵抗中的作用。这种方法没有假设 哪些基因会导致补体抵抗， 鉴定以前未被识别的基因产物，这些基因产物在这一过程中发挥作用。 过程