RESTRICTION FRAGMENT LENGTH POLYMORPHISMS IN NORMAL AND NEOPLASTIC TISSUES

正常和肿瘤组织中的限制性片段长度多态性

• 批准号: 3808537
• 负责人: B ZBAR
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3808537
• 关键词: cardiovascular neoplasm; chromosome walking; genetic library; genetic mapping; genetic markers; head /neck neoplasm; human genetic material tag; human population genetics; human subject; human tissue; linkage mapping; molecular cloning; neoplasm /cancer genetics; nucleic acid probes; restriction fragment length polymorphism; restriction mapping; retina disorder; small cell lung cancer; squamous cell carcinoma

The major goal of the research effort was to precisely locate the von Hippel-Lindau disease gene. Previous studies from our laboratory indicated that VHL is located in a 6-8 cM interval between RAF1 and D3Sl8. D3S601 is the closest marker we have identified to the VHL gene; it is located in a region immediately surrounding the VHL gene. We have isolated YAC clones for all markers close to the VHL locus and are in the process of cloning the region by YAC-based chromosome walking. Forty-three probes homozygously deleted in a small cell lung carcinoma line were identified. Human squamous cell carcinomas of the head and neck were shown to be characterized by a loss of alleles at loci on chromosome 3p.

研究工作的主要目标是精确定位von