RESTRICTION FRAGMENT LENGTH POLYMORPHISMS IN NORMAL AND NEOPLASTIC TISSUES

正常和肿瘤组织中的限制性片段长度多态性

• 批准号: 5200958
• 负责人: B ZBAR
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5200958
• 关键词: bioassay; carcinoma; family genetics; genetic mapping; genetic markers; human subject; kidney neoplasms; molecular oncology; neoplasm /cancer genetics; pediatric neoplasm /cancer; pheochromocytoma; restriction fragment length polymorphism; small cell lung cancer; transfection

The major goal of the research project is to identify and characterize genes involved in the pathogenesis of kidney cancer and small cell carcinoma of the lung. Our major accomplishments this year are: 1.The identification of another germline missense VHL mutation (547 T to C) associated with frequent pheochromocytomas without renal cell carcinoma. 2.The demonstration that VHL gene mutations that produce certain tumors in Western VHL families produced the same tumor spectrum in Japanese VHL families. 3.The detection of the VHL protein, pVHL/vhilin, in Cos 7 cells transfected with the VHL cDNA. The detection of the VHL protein in the nucleus of renal carcinoma cells. 4.The development of a biological assay that demonstrates the growth suppressive effects of the VHL gene in renal carcinoma cells. 5.The identification of a large Canadian family with papillary renal cell carcinoma.

该研究项目的主要目标是识别和表征 参与肾癌和小细胞肺癌发病机制的基因 肺癌 我们今年的主要成就是： 1.另一个VHL错义突变（547 T）的鉴定 至C）与无肾细胞的频繁嗜铬细胞瘤相关 carcinoma. 2.证明VHL基因突变产生某些 Western VHL家族中的肿瘤产生了相同的肿瘤谱， 日本VHL家族 3. Cos 7细胞中VHL蛋白pVHL/vhilin的检测 用VHL cDNA转染。 VHL蛋白的检测 肾癌细胞核。 4.开发一种生物测定法， VHL基因对肾癌细胞的抑制作用。 5.一个加拿大乳头状肾大家族的鉴定 细胞癌