MOLECULAR STRUCTURE OF ANIMAL VIRUSES AND CELLS BY COMPUTATIONAL ANALYSIS

通过计算分析动物病毒和细胞的分子结构

• 批准号: 3813366
• 负责人: J V MAIZEL
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3813366
• 关键词: Adenoviridae; Picornaviridae; RNA splicing; computer assisted sequence analysis; computer data analysis; computer graphics /printing; computer program /software; computer simulation; conformation; electron microscopy; genetic promoter element; genetic recombination; glycoproteins; human immunodeficiency virus 1; macromolecule; mathematical model; molecular dynamics; nucleic acid sequence; protein sequence; protein structure function; virus RNA; virus morphology; virus protein; virus replication

Viral systems are used to model the complex macromolecular processes and organization of normal, infected and transformed cells. Computers are used to study the nucleic acid and protein sequences that embody the information necessary for these systems to function. Picornaviruses cause diseases typified by polio, colds, hepatitis, and foot-and-mouth diseases. Gene and protein sequences of these viruses are comparatively studied for relationships among them and to other known and hypothetical proteins. Secondary structures of the RNAs are found to vary in correlation with pathological and sequence differences. Adenoviruses are studied with a goal to understanding early events in virus replication wherein the cell's metabolism is subverted to viral functions, and late events during which assembly and morphogenesis occurs. EarLy viral proteins, whose existence was known from biochemical studies, have been analyzed by comparing their sequences to cellular proteins of known function. Computer analyses of proteins and nucleic acids have been developed and implemented in conjunction with techniques of biochemistry, virology, and electron microscopy on sequences of picornaviruses, adenoviruses, human immunodeficiency viruses. Graphic representations revealing homology, and reverse complementarity are coupled with numerical methods to aid the prediction of secondary structure, splicing, promoters, and recombination in nucleic acid molecules. Computer programs are developed locally and elsewhere for application on Cray XMP, VAX and graphic workstations to perform sequence analysis and structure predictions. Methods to assess the significance of predictions use Monte Carlo simulations, evolutionary comparisons and biochemical data. Roles for genes and proteins are deduced by comparison with databases of sequences of known function and structure.

病毒系统用于模拟复杂的大分子过程 正常细胞、感染细胞和转化细胞的组织。使用电脑 研究体现信息的核酸和蛋白质序列 这些系统正常运行所必需的。 小核糖核酸病毒引起的疾病包括脊髓灰质炎、感冒、肝炎和 手足口病。这些病毒的基因和蛋白质序列是 对它们之间以及与其他已知和其他已知的关系进行比较研究 假设的蛋白质。发现 RNA 的二级结构有所不同 与病理和序列差异相关。 研究腺病毒的目的是了解病毒的早期事件 复制，其中细胞的新陈代谢被颠覆为病毒功能， 以及发生组装和形态发生的晚期事件。早期病毒 蛋白质的存在已从生化研究中得知， 通过将其序列与已知的细胞蛋白质进行比较来进行分析 功能。 蛋白质和核酸的计算机分析已经开发出来 与生物化学、病毒学等技术结合实施 电子显微镜观察小核糖核酸病毒、腺病毒、人类 免疫缺陷病毒。揭示同源性的图形表示，以及 反向互补与数值方法相结合，以帮助 二级结构、剪接、启动子和重组的预测 在核酸分子中。计算机程序是在本地开发的 其他地方可在 Cray XMP、VAX 和图形工作站上应用 进行序列分析和结构预测。评估方法 预测的重要性使用蒙特卡罗模拟、进化 比较和生化数据。推断基因和蛋白质的作用 通过与已知功能和结构的序列数据库进行比较。