MOLECULAR STRUCTURE OF ANIMAL VIRUSES AND CELLS BY COMPUTATIONAL ANALYSIS

小核糖核酸病毒会引起以小儿麻痹症、感冒、肝炎和 口蹄疫。已经对这些病毒的序列进行了检查 它们之间的关系以及与其他已知和假设的蛋白质的关系。 已经发现RNA的二级结构相对于 病理和序列变异。 研究腺病毒的目的是为了了解病毒的早期事件。 细胞的新陈代谢被病毒功能颠覆的复制， 以及在组装和形态发生过程中发生的晚期事件。早些时候 从生化研究中得知其存在的病毒蛋白有 通过将它们的序列与已知的细胞蛋白质进行比较来进行分析 功能。病毒E3糖蛋白与病毒的物理联系 细胞MHC蛋白与其序列之间的同源性相关。 生物化学、病毒学、电子显微镜和计算机技术 分析用于研究微小核糖核酸病毒和腺病毒。分析了 蛋白质和核酸已经被开发和实施。图解 揭示同源性和反向互补的表示是耦合的 用于辅助预测二级结构、剪接、 启动子，以及核酸分子中的重组。计划是 在为SEQUENCE设计的VAX 11/750系统上开发和安装 分析。已经预测了多达2000个基地的结构。方法，以 使用蒙特卡罗模拟评估预测的重要性， 进化比较和生化数据。蛋白质二级结构 是从氨基酸序列预测出来的。比较新的序列 使用计算机化的数据库来检测与已知蛋白质的关系。 呼肠孤病毒蛋白Sigma-1已被发现具有七肽模式 典型的盘绕线圈，阿尔法螺旋结构。

Picornaviruses cause disease typified by polio, colds, hepatitis, and foot-and-mouth diseases. Sequences of these viruses have been examined for relationships among them and to other known and hypothetical proteins. Secondary structures of the RNAs have been found to vary with respect to pathological and sequences variances. Adenoviruses are studied with a goal to understanding early events in virus replication wherein the cell's metabolism is subverted to viral functions, and late events during which assembly and morphogenesis occurs. Early viral proteins, whose existence was known from biochemical studies, have been analyzed by comparing their sequences to cellular proteins of known function. Physical association between the viral E3 glycoprotein and cellular MHC proteins correlates with homology between their sequences. Techniques of biochemistry, virology, electron microscopy and computer analysis are used to study picornaviruses and adenoviruses. Analyses of proteins and nucleic acids have been developed and implemented. Graphic representations revealing homology, and reverse complementarity are coupled numerical methods to aid the prediction of secondary structure, splicing, promoters, and recombination in nucleic acid molecules. Programs are developed and installed in a VAX 11/750 system designed for sequence analysis. Structures of up to 2000 bases have been predicted. Methods to assess the significance of predictions use Monte Carlo simulations, evolutionary comparisons and biochemical data. Protein secondary structure is being predicted from amino acid sequences. New sequences are compared with computerized databases to detect relationships with known proteins. Reovirus protein sigma-1 has been found to have a heptapeptide pattern typical of a coiled-coil, alpha-helical structure.