MOLECULAR STRUCTURE OF ANIMAL VIRUSES AND CELLS BY COMPUTATIONAL ANALYSIS

通过计算分析动物病毒和细胞的分子结构

• 批准号: 3963024
• 负责人: J V MAIZEL
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3963024
• 关键词: Adenoviridae; Picornaviridae; computer data analysis; computer graphics /printing; computer simulation; conformation; electron microscopy; genetic recombination; major histocompatibility complex; mathematical model; nucleic acid sequence; virus RNA; virus morphology; virus replication

Picornaviruses cause disease typified by polio, colds, hepatitis, and foot-and-mouth diseases. Sequences of these viruses have been examined for relationships among them and to other known and hypothetical proteins. Secondary structures of the RNAs have been found to vary with respect to pathological and sequences variances. Adenoviruses are studied with a goal to understanding early events in virus replication wherein the cell's metabolism is subverted to viral functions, and late events during which assembly and morphogenesis occurs. Early viral proteins, whose existence was known from biochemical studies, have been analyzed by comparing their sequences to cellular proteins of known function. Physical association between the viral E3 glycoprotein and cellular MHC proteins correlates with homology between their sequences. Techniques of biochemistry, virology, electron microscopy and computer analysis are used to study picornaviruses and adenoviruses. Analyses of proteins and nucleic acids have been developed and implemented. Graphic representations revealing homology, and reverse complementarity are coupled numerical methods to aid the prediction of secondary structure, splicing, promoters, and recombination in nucleic acid molecules. Programs are developed and installed in a VAX 11/750 system designed for sequence analysis. Structures of up to 2000 bases have been predicted. Methods to assess the significance of predictions use Monte Carlo simulations, evolutionary comparisons and biochemical data. Protein secondary structure is being predicted from amino acid sequences. New sequences are compared with computerized databases to detect relationships with known proteins. Reovirus protein sigma-1 has been found to have a heptapeptide pattern typical of a coiled-coil, alpha-helical structure.

小核糖核酸病毒引起以脊髓灰质炎、感冒、肝炎和 口蹄疫 这些病毒的序列已被检查， 它们之间的关系以及与其他已知和假设的蛋白质的关系。 已发现RNA的二级结构随 病理和序列差异。 研究腺病毒的目的是了解病毒感染的早期事件， 复制，其中细胞的代谢被破坏为病毒功能， 以及在其期间发生组装和形态发生的晚期事件。 早期 病毒蛋白，其存在是已知的从生化研究， 通过将它们的序列与已知的细胞蛋白质进行比较， 功能 病毒E3糖蛋白与 细胞MHC蛋白与它们的序列之间的同源性相关。 生物化学、病毒学、电子显微镜和计算机技术 分析用于研究小核糖核酸病毒和腺病毒。 分析 蛋白质和核酸已经被开发和应用。 图形 显示同源性的表示和反向互补性被偶联 数值方法来帮助预测二级结构，剪接， 启动子和核酸分子中的重组。 程序 开发并安装在VAX 11/750系统中， 分析. 已经预测了多达2000个碱基的结构。 方法 使用蒙特卡罗模拟评估预测的重要性， 进化比较和生化数据。 蛋白质二级结构 是从氨基酸序列中预测出来的 比较新序列 利用计算机化数据库来检测与已知蛋白质的关系。 已发现呼肠孤病毒蛋白sigma-1具有七肽模式 典型的卷曲螺旋、α-螺旋结构。