IDENTIFICATION OF OXACILLIN RESISTANT STRAINS OF STAPHYLOCOCCUS AUREUS

金黄色葡萄球菌苯唑西林耐药菌株的鉴定

• 批准号: 3874362
• 负责人: C H ZIERDT
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3874362
• 关键词: Staphylococcus aureus; bacterial virus; beta lactam antibiotic; beta lactamase; genetic strain; human tissue; microorganism culture; nosocomial infections

A multi-center study of oxacillin resistant S. aureus is in place and should be completed within a year. Phage types have been, and will continue to be used to fingerprint strains of the oxacillin resistant species. We have evidence that strains with oxacillin resistance not reversible by clavulanic acid are few in number, and that oxacillin resistant S. aureus is represented in many U.S. hospitals by a single predominant strain. S. aureus in which oxacillin resistance is reduced in the presence of clavulanic acid, the hyperbetalactamase producers, have another, distinct phage pattern. We also are studying loss rates of oxacillin resistance after the strains are cultured from the treated patient. This phase will include initial sensitivity tests done directly from clinical samples, followed by tests done during serial subcultures. This has important bearing on sensitivity testing of these S. aureus strains, since they begin to lose resistance immediately, on initial cultures.

一项针对苯唑西林耐药金黄色葡萄球菌的多中心研究已经到位，并且 应在一年内完成。噬菌体类型已经并将继续 用于对苯唑西林抗性物种进行指纹识别。我们 有证据表明具有苯唑西林耐药性的菌株不可逆转 克拉维酸数量很少，且对苯唑西林具有抗药性的金黄色葡萄球菌 在许多美国医院中，该菌株以单一主要菌株为代表。 S。 金黄色葡萄球菌，其中苯唑西林耐药性在存在时降低 克拉维酸，超β内酰胺酶的产生者，还有另一个独特的作用 噬菌体模式。我们还在研究苯唑西林耐药性的丧失率 从接受治疗的患者体内培养出菌株后。这一阶段将 包括直接从临床样本进行的初始敏感性测试， 随后在连续传代培养期间进行测试。这有重要的 影响这些金黄色葡萄球菌菌株的敏感性测试，因为它们开始 立即失去对初始培养物的抵抗力。