CULTIVATION OF MYCOBACTERIUM LEPRAE

M的繁殖。麻风病已经在体外获得，并持续了 每隔30-60天传代9次。 每毫升最好的数字 令人失望，稳定在每毫升1x 106 - 1x 107。 可能 有必要开发一种连续生长的大容量发酵罐 系统来获取毫克或克数量的湿或干生长。 增长 使用佐剂试图增加生长速率， 以及细胞收获。 一种新的分枝杆菌，一种“犰狳衍生的分枝杆菌” (ADM)也长大了。 我们可以很快地将它与M区分开来。 通过吡啶脱色，使M.非酸性麻风 快了 ADM现在已经完全特征化。 根据生理学， 基因和抗原研究表明，这是一个新物种。 最终实验 对麻风分枝杆菌体外培养的影响。 斯科特 卡维尔的汉森疾病中心的弗兰兹布劳是一个合作者 本研究 他一直在为我们提供M。麻风定形细胞 测试物种。 我们可以接触到M麻风病的物质来自 受感染的犰狳（肝脏）。 Armadillo-derived M.麻风病人总是 犰狳衍生的分枝杆菌或ADM的混合物。 其中有20种，没有名字。 的ADM 过度生长M.在我们的文化技术中， Scott Franzblau的M.麻风 受感染的裸鼠群最近被一场动物流行病消灭了。 这些老鼠需要一年的时间来发展感染。 我们必须等这么久 完成我们的实验 进展：我们现在准备与Scott Franzblau合作， 完成生长实验。 裸小鼠M leprae材料 再次从Franzblau博士处获得。 增长的问题， 这个独特的方法将在几个月内决定。 即使没有 增长，该技术仍然有希望， 出版以引起他人的兴趣。

Multiplication of M. leprae has been obtained in vitro, and continued for nine passages at 30-60 day intervals. Best numbers per milliliter have been disappointing, leveling off at 1x106-1x107 per milliliter. It may be necessary to develop a continuous growth, large volume fermentor system to acquire milligram or gram amounts of wet or dry growth. Growth adjuvants are being used in an attempt to increase the growth rate as well as the cell harvest. A new species of mycobacterium, an "armadillo-derived mycobacterium" (ADM), has also grown out. We can quickly differentiate it from M. leprae by pyridine decolorization, which renders M. leprae, non-acid- fast. The ADM has now been fully characterized. According to physiological, genetic, and antigenic studies, it is a new species. Final experiments on the in vitro culture of Mycobacterium leprae are delayed. Scott Franzblau of the Hansen's disease center at Carville is a collaborator in this study. He has been supplying us with M. leprae cells definitive tests for the species. We have access to M. leprae material from infected armadillos (the liver). Armadillo-derived M. leprae always has an admixture of armadillo-derived mycobacteria, or ADM. There are about twenty species of these, which have not been given names. The ADM overgrow M. laprae in our culture technique. Scott Franzblau's M. leprae infected nude mouse colony was recently wiped out with an epizootic. These mice take a year to develop infections. We must wait this long to finish our experiment. Progress: We are ready now, in collaboration with Scott Franzblau, to complete the growth experiments. Nude mouse M leprae material is available once again from Dr. Franzblau. The question of growth using this unique method will be decided within a few months. Even if no growth is demonstrated, the technique would still have promise and would be published to interest others in its continuation.