CULTIVATION OF MYCOBACTERIUM LEPRAE

麻风分枝杆菌的培养

• 批准号: 3916517
• 负责人: C H ZIERDT
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916517
• 关键词: Mycobacterium leprae; biotechnology; glycolipids; growth media; kanamycin; leprosy; microorganism antigen; microorganism culture; microorganism genetics; microorganism mass culture; pyridine; rifamycins; streptomycin; tetracyclines

Multiplication of M. leprae has been obtained in vitro, and continued for nine passages at 30-60 day intervals. Best numbers per milliliter have been disappointing, leveling off at 1x10-6 - 1x10-7 per milliliter. It may be necessary to develop a continuous growth, large volume fermentor system to acquire milligram or gram amounts of wet or dry growth. Growth adjuvants are being used in an attempt to increase the growth rate as well as the cell harvest. We were unable to duplicate the culture results of Murohashi and Yoshida; our results using their procedures were completely negative. A new species of mycobacterium, an "armadillo-derived mycobacterium" (ADM), has also grown out. We can quickly differentiate it from M. leprae by pyridine decolorization, which renders M. leprae, but not the ADM, non-acid-fast. The ADM has been found susceptible to kanamycin, amikacin, streptomycin, rifampin, and tetracycline. We are using kanamycin at 10 mcg/ml to eliminate it from our M. leprae cultures.

M的繁殖。麻风病已经在体外获得，并持续了 每隔30-60天传代9次。 每毫升最好的数字 一直令人失望，稳定在每毫升1x 10 -6 - 1x 10 -7。 可能 有必要开发连续生长、大体积发酵罐系统 获得毫克或克数量的湿或干生长。 增长 也在尝试使用辅助剂来提高生长速率 随着细胞的收获。 我们无法复制 Murohashi和Yoshida;我们使用他们的程序的结果完全正确。 阴性 一种新的分枝杆菌，一种“犰狳衍生的分枝杆菌”（ADM）， 也长大了。 我们可以很快地将它与M区分开来。麻风病比 吡啶脱色，使M.麻风病，但不是ADM 不耐酸。 ADM对卡那霉素、丁胺卡那霉素、链霉素敏感， 利福平和四环素 我们使用10 mcg/ml的卡那霉素， 把它从我们的M中去掉麻风菌培养物。