CULTIVATION OF MYCOBACTERIUM LEPRAE

麻风杆菌的体外增殖已经获得，并继续进行了 每隔30-60天进行9次传代。每毫升最好的数字 一直令人失望，稳定在每毫升1x10-6-1x10-7。它可能 有必要开发一个连续增长的大容量发酵罐系统 获得毫克或克量的湿或干生长。生长 使用佐剂也是为了提高生长速度。 随着细胞的收获。我们无法复制的文化成果 Murohashi和Yoshida；我们使用他们的程序得到的结果完全是 不是。 分枝杆菌的一个新种，一种“犰螂衍生的分枝杆菌”(ADM)， 也已经成长起来。我们可以通过以下方法迅速将其与麻风杆菌区分开来 吡啶脱色，产生麻风杆菌，而不是ADM， 不耐酸。 已发现ADM对卡那霉素、阿米卡星、链霉素、 利福平和四环素。我们正在使用10微克/毫升的卡那霉素 把它从我们的麻风杆菌培养中消灭。

Multiplication of M. leprae has been obtained in vitro, and continued for nine passages at 30-60 day intervals. Best numbers per milliliter have been disappointing, leveling off at 1x10-6 - 1x10-7 per milliliter. It may be necessary to develop a continuous growth, large volume fermentor system to acquire milligram or gram amounts of wet or dry growth. Growth adjuvants are being used in an attempt to increase the growth rate as well as the cell harvest. We were unable to duplicate the culture results of Murohashi and Yoshida; our results using their procedures were completely negative. A new species of mycobacterium, an "armadillo-derived mycobacterium" (ADM), has also grown out. We can quickly differentiate it from M. leprae by pyridine decolorization, which renders M. leprae, but not the ADM, non-acid-fast. The ADM has been found susceptible to kanamycin, amikacin, streptomycin, rifampin, and tetracycline. We are using kanamycin at 10 mcg/ml to eliminate it from our M. leprae cultures.