QUANTITATIVE ANALYSIS OF HIV-1 PROVIRAL DNA IN SAMPLES FROM PATIENTS WITH AIDS

艾滋病患者样本中 HIV-1 前病毒 DNA 的定量分析

• 批准号: 3874497
• 负责人: H MITSUYA
• 金额: --
• 依托单位: DIVISION OF CANCER TREATMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3874497
• 关键词: AIDS; AIDS therapy; HIV infections; antiviral agents; human immunodeficiency virus 1; human subject; inosine; longitudinal human study; monocyte; polymerase chain reaction; provirus; virus DNA

A rapid and simple method for determining the proviral DNA content in peripheral blood mononuclear cells (PBM) from patients with human immunodeficiency virus type I (HIV-1) infection was established by using the polymerase chain reaction (PCR) technique. We found that the majority of HIV proviral DNA copies detectable in unfractionated PBM resided in T cells, while B cells/monocytes contained lesser amounts of HIV DNA (93.9+- 3.5% for T cells versus 6.1+-3.5% for B cells/monocytes: p<0.05). When we compared the amount of HIV proviral DNA in PBM from 13 patients with AIDS or ARC before and during antiretroviral therapy with 2',3'dideoxyinosine (ddI) which was given as an escalating dose in a phase I clinical study, a significant decrease was observed in 9 out of 12 evaluable patients receiving the drug for 8 to 14 weeks (p<0.02). The decrease appeared more pronounced in patients receiving relatively high doses of the drug. These data suggest that the quantitation of HIV viral DNA in PBM by PCR is feasible and may theoretically contribute to an overall monitoring of patients receiving experimental therapy. However, larger studies will be required to determine the sensitivity and specificity of this assay and further longitudinal studies will be essential.

一种快速简便的测定原病毒DNA含量的方法