ISOLATION OF GENES DIFFERENTALLY EXPRESSED IN BRAIN OF OLD AND YOUNG RATS

尽管据报道发生了结构和生化变化 在衰老的大脑中，衰老过程背后的机制(S) 它本身仍然是未知的。因此，我们开始了一个新的项目 旨在探索老年人和年轻人的差异基因表达 老鼠的大脑。使用减法杂交技术，我们可以 试图克隆差异表达的基因 老龄(24月龄)和成熟青年(5月龄)大鼠。简而言之， 基本的方法是构建两个cdna文库；一个来自Poly A+RNA来自幼年大鼠，另一种来自老年大鼠聚A+RNA 老鼠。将来自同一年龄组的Poly A+RNA与cDNA杂交 来自相反年龄组和cDNAm RNA杂交体 已删除。结果，获得了非杂交的cDNA和 随后包装在细菌载体中，应进行浓缩或 高度特定于特定年龄段的人。最终，这将是 让我们有机会识别和研究那些 与大脑的衰老过程有关 他们增加的表达(或者相反，通过他们的亲戚 不活动)在特定年龄。

Despite the structural, and biochemical changes reported to occur in aging brain, the mechanism(s) underlying the aging process itself remain unknown. Thus, we have begun a new project designed to explore differential gene expression in old and young rat brain. Using subtraction hybridization techniques we are attempting to clone genes which are differentially expressed in aged (24 month) and mature young (5 month) rats. Briefly, the basic approach is to construct two cDNA libraries; one from poly A+ RNA from young rats, the other from poly A+ RNA from old rats. Poly A+ RNA from one age group is hybridized to the cDNA derived from the opposite age group and the cDNA/mRNA hybrids removed. AS a result, nonhybridizing cDNAs obtained and subsequently package in a bacterial vector, should be enriched or highly specific for a particular age group. Ultimately, this will give us the opportunity to identify and study those genes which are involved (associated) with the aging process in brain by virtue of their increased expression (or conversely, by their relative inactivity) at specific ages.