THREE-DIMENSIONAL HISTOLOGICAL RECONSTRUCTION

一种半自动三维结构采集系统 有关组织学材料的信息正在开发中。系统 应该比使用串口的技术更快、更可靠 各节，尽管决议可能受到限制。简而言之，嵌入的 组织块将相对于扫描电子显微镜进行固定 成像系统；块的表面将被成像，图像 储存，连续的切片将被内置的切片机取出。 因此，薄片的处理和对准将被取消。 人和计算机的模式识别将转换结果集 将图像转化为三维重建。照片中的 通过这种技术获得的粗角赫米森达与 相同组织的电子显微镜图像。

A semiautomatic system for acquiring three-dimensional structural information about histological material is being developed. The system should be faster and more reliable than techniques that use serial sections, although resolution may be limited. In brief, an embedded tissue block will be fixed relative to a scanning electron microscope imaging system; the surface of the block will be imaged and the image stored, and successive slices will be removed by a built-in microtome. Handling and registration of thin sections will thus be eliminated. Human and computer pattern recognition will transform the resulting set of images into a three-dimensional reconstruction. The images of Hermissenda crassicornis obtained by this technique correlate well with TEM images of the same tissue.