THREE-DIMENSIONAL HISTOLOGICAL RECONSTRUCTION

三维结构半自动采集系统 关于组织学材料的信息正在开发中。 系统 与现有技术相比， 使用连续切片的技术，尽管分辨率可能有限。 在 简而言之，包埋的组织块将相对于扫描被固定， 电子显微镜成像系统，块的表面将被成像 和图像存储，连续切片将被删除的内置 切片机 因此，薄切片的处理和配准将 淘汰 人类和计算机模式识别将改变 将所得到的图像集转化为三维重建。 氧 已经发现等离子体蚀刻可以提供足够的地形起伏， 图像的分辨率现在受到SEM的限制，而不是受到 制备技术 赫米森达Crassicornis获得的图像， 该技术与相同组织的TEM图像很好地相关， 这表明缺乏伪影对于预期的 问题研究

A semi-automatic system for acquiring three-dimensional structural information about histological material is being developed. The system should have significant speed and reliability advantages over present techniques using serial sections, although resolution may be limited. In brief, an embedded tissue block will be fixed relative to a scanning electron microscope imaging system, the surface of the block will be imaged and the image stored, and successive slices will be removed by a built-in microtome. Handling and registration of thin sections will thus be eliminated. Human and computer pattern recognition will transform the resulting set of images into a three-dimensional reconstruction. Oxygen plasma etching has been found to give sufficient topographic relief that the resolution of the images is now limited by the SEM and not by the preparation technique. The images of Hermissenda Crassicornis obtained by this technique correlate well with TEM images of the same tissue, indicating that the lack of artifact is adequate for the contemplated studies.