THREE-DIMENSIONAL HISTOLOGICAL RECONSTRUCTION

三维结构半自动采集系统 关于组织学材料的信息正在开发中。系统 应该比使用串行通信的技术更快、更可靠。 部分，虽然分辨率可能有限。简而言之， 将块相对于扫描电子显微镜固定成像 系统中，块的表面将被成像并存储图像，以及 将通过内置切片机去除连续切片。处理和 因此将消除薄切片的配准。人机 模式识别将把得到的图像集转换成 三维重建已经发现氧等离子体蚀刻 提供足够的地形起伏， 受SEM的限制而不受制备技术的限制。的图像 用该技术获得的厚角赫米森达线虫（Hermissenda Crassicornis）与透射电镜（TEM）结果吻合较好 相同组织的图像。

A semi-automatic system for acquiring three-dimensional structural information about histological material is being developed. The system should be faster and more reliable than techniques that use serial sections, although resolution may be limited. In brief, an embedded tissue block will be fixed relative to a scanning electron microscope imaging system, the surface of the block will be imaged and the image stored, and successive slices will be removed by a built-in microtome. Handling and registration of thin sections will thus be eliminated. Human and computer pattern recognition will transform the resulting set of images into a three-dimensional reconstruction. Oxygen plasma etching has been found to give sufficient topographic relief that the resolution of the images is now limited by the SEM and not by the preparation technique. The images of Hermissenda Crassicornis obtained by this technique correlate well with TEM images of the same tissue.