THREE-DIMENSIONAL HISTOLOGICAL RECONSTRUCTION

三维结构半自动采集系统 关于组织学材料的信息正在开发中。 的 系统应该比使用 连续切片，尽管分辨率可能有限。 简而言之， 包埋的组织块将相对于扫描电子 显微镜成像系统，块的表面将被成像 和图像存储，连续切片将被删除， 内置切片机 薄切片的处理和登记 将因此被淘汰。 人机模式识别 会将生成的图像集转换为三维图像 重建 已发现氧等离子体蚀刻给出 足够的地形起伏，图像的分辨率 现在受SEM限制，而不受制备技术限制。 的 通过这种技术获得的Hermissenda Crassicornis的图像 与相同组织的TEM图像很好地相关。

A Semi-automatic system for acquiring three-dimensional structural information about histological material is being developed. The system should be faster and more reliable than techniques that use serial sections, although resolution may be limited. In brief, an embedded tissue block will be fixed relative to a scanning electron microscope imaging system, the surface of the block will be imaged and the image stored, and successive slices will be removed by a built-in microtome. Handling and registration of thin sections will thus be eliminated. Human and computer pattern recognition will transform the resulting set of images into a three-dimensional reconstruction. Oxygen plasma etching has been found to give sufficient topographic relief that the resolution of the images is now limited by the SEM and not by the preparation technique. The images of Hermissenda Crassicornis obtained by this technique correlate well with TEM images of the same tissue.